Shenzhen Aier Eye Hospital, Aier Eye Hospital, Jinan University, Shenzhen, China.
Shenzhen Aier Eye Hospital, Aier Eye Hospital, Jinan University, Shenzhen, China; Shenzhen Aier Ophthalmic Technology Institute, Shenzhen, China; School of Life and Health Sciences, The Chinese University of Kong Hong, Shenzhen 518000, Guangdong, China.
Ageing Res Rev. 2023 Sep;90:102030. doi: 10.1016/j.arr.2023.102030. Epub 2023 Aug 5.
Age-related macular degeneration (AMD) is the leading cause of blindness in individuals over the age of 50 years, yet its etiology and pathogenesis largely remain uncovered. Single-cell RNA sequencing (scRNA-seq) technologies are recently developed and have a number of advantages over conventional bulk RNA sequencing techniques in uncovering the heterogeneity of complex microenvironments containing numerous cell types and cell communications during various biological processes. In this review, we summarize the latest discovered cellular components and regulatory mechanisms during AMD development revealed by scRNA-seq. In addition, we discuss the main challenges and future directions in exploring the pathophysiology of AMD equipped with single-cell technologies. Our review underscores the importance of multimodal single-cell platforms (such as single-cell spatiotemporal multi-omics and single-cell exosome omics) as new approaches for basic and clinical AMD research in identifying biomarker, characterizing cellular responses to drug treatment and environmental stimulation.
年龄相关性黄斑变性（AMD）是 50 岁以上人群失明的主要原因，但该病的病因和发病机制在很大程度上仍未被揭示。单细胞 RNA 测序（scRNA-seq）技术是最近开发的，与传统的批量 RNA 测序技术相比，它在揭示各种生物过程中包含多种细胞类型和细胞通讯的复杂微环境异质性方面具有许多优势。在这篇综述中，我们总结了 scRNA-seq 揭示的 AMD 发展过程中最新发现的细胞成分和调控机制。此外，我们还讨论了利用单细胞技术探索 AMD 病理生理学的主要挑战和未来方向。我们的综述强调了多模式单细胞平台（如单细胞时空多组学和单细胞外泌体组学）的重要性，这些平台是用于确定生物标志物、描述细胞对药物治疗和环境刺激的反应的基础和临床 AMD 研究的新方法。
