Tosh R E, Collings P J
Biochim Biophys Acta. 1986 Jul 10;859(1):10-4. doi: 10.1016/0005-2736(86)90312-3.
The one previously reported high pressure volumetric experiment on a phospholipid bilayer investigated a region of pressure between 0 and 25 MPa and obtained isothermal compressibility values for the liquid crystal and intermediate phases which differed by more than a factor of ten. We report new volumetric measurements around the main transition in dipalmitoylphosphatidylcholine (DPPC) from 0 to 100 MPa. The isothermal compressibility data for the two phases are of the same order of magnitude, and the experimentally determined coexistence curve, specific volume dependence, and volume discontinuity values are compared with the predictions of the phenomenological theory according to Sugar and Tarjan ((1982) Sov. Phys. Crystallogr. 27, 4-5). Significant discrepancies between this theory and experiment are found. Finally, the data indicate that steric interactions play a more dominant role in the main transition of phospholipid bilayers than in transitions in most thermotropic liquid crystals.
之前报道的一项关于磷脂双层的高压体积实验研究了0至25兆帕的压力范围，并获得了液晶相和中间相的等温压缩率值，二者相差超过十倍。我们报告了围绕二棕榈酰磷脂酰胆碱（DPPC）主转变点从0至100兆帕的新体积测量结果。两相的等温压缩率数据处于同一数量级，并且将实验测定的共存曲线、比容依赖性和体积不连续值与根据苏加尔和塔尔扬（（1982年）《苏联物理晶体学》27卷，4 - 5页）的唯象理论预测值进行了比较。发现该理论与实验之间存在显著差异。最后，数据表明空间相互作用在磷脂双层的主转变中比在大多数热致液晶的转变中发挥着更主导的作用。
