Department of Ophthalmology, Edward S. Harkness Eye Institute, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA.
Department of Biophysics, Faculty of Medicine, Gazi University, Ankara, Turkey.
Transl Vis Sci Technol. 2023 Aug 1;12(8):4. doi: 10.1167/tvst.12.8.4.
The purpose of this study was to quantify retinal hydration (RH) levels with optical coherence tomography (OCT) and determine the extent of cellular damage resulting from intraretinal fluid alterations.
We took 6.0 mm sections of the human sensory retina that were excised from 18 fresh (<24 hours) donor eyes. They were either exposed to various osmotic stresses between 90 and 305 mOsm or dehydrated under a laminar flow hood. Change in tissue weight was used to calculate the retinal water content (RWC). Image analyses were conducted on OCT between 0 and 180 minutes to assess retinal thickness (RT) and "optically empty areas" (OEAs) representing intraretinal fluid. Correlations were sought among RWC, OEA, RWC, and RT. The effect of RH on retinal cell viability (RCV) was assessed with the Live-Dead Assay.
RH demonstrated a stronger correlation with the OEA than plain RT measurements (r = 0.99, P < 0.001). RH-RCV interaction fits well to a bell-shaped curve. A significant proportion of retinal cells (>80%) remained viable despite the change in RH ranging between 0.87 and 1.42 times. This "safe zone" was found to be associated with a 22% increase in OEA (r = 0.99, P < 0.01).
OCT has been demonstrated as a valuable tool for assessing RH and can be used for intraretinal fluid content analysis. RH is a better indicator of RCV compared with RT. Computing RH may improve the determination of functional outcome of intravitreal pharmacotherapeutics used for diabetic macular edema and exudative age-related macular degeneration.
We link basic research and clinical care by assessing retinal hydration's impact on retinal fluid dynamics, macular edema, and cell viability.
本研究旨在通过光相干断层扫描（OCT）定量评估视网膜水合（RH）水平，并确定细胞损伤的程度与视网膜内液改变之间的关系。
我们从 18 只新鲜（<24 小时）供体眼球中取出 6.0mm 节段的人感觉视网膜。将它们暴露于 90 至 305mOsm 之间的各种渗透压应激下，或在层流罩下脱水。组织重量的变化用于计算视网膜水含量（RWC）。在 0 至 180 分钟之间进行 OCT 图像分析，以评估视网膜厚度（RT）和代表视网膜内液的“光学空区”（OEAs）。寻找 RWC、OEA、RWC 和 RT 之间的相关性。通过 Live-Dead 测定评估 RH 对视网膜细胞活力（RCV）的影响。
RH 与 OEA 的相关性强于普通 RT 测量（r=0.99，P<0.001）。RH-RCV 相互作用拟合良好呈钟形曲线。尽管 RH 变化在 0.87 至 1.42 倍之间，超过 80%的视网膜细胞仍保持活力。该“安全区”与 OEA 增加 22%相关（r=0.99，P<0.01）。
OCT 已被证明是评估 RH 的一种有价值的工具，可用于分析视网膜内液含量。与 RT 相比，RH 是 RCV 的更好指标。计算 RH 可能会提高评估用于糖尿病性黄斑水肿和渗出性年龄相关性黄斑变性的玻璃体内药物治疗的功能结果的准确性。
翻译后的文本已去除了英文原文和其他说明性文字，只保留了需要翻译的内容。
