Farinha Cláudia, Santos Torcato, Marques Inês P, Marques João P, Ribeiro Luísa, Figueira João, Silva Rufino, Cunha-Vaz José
Association for Innovation and Biomedical Research on Light and Image, Coimbra, Portugal; Department of Ophthalmology, Centro Hospitalar e Universitário de Coimbra (CHUC), Coimbra, Portugal.
Association for Innovation and Biomedical Research on Light and Image, Coimbra, Portugal.
Ophthalmol Retina. 2017 Nov-Dec;1(6):486-496. doi: 10.1016/j.oret.2017.03.004. Epub 2017 May 10.
To test OCT-Leakage, a new method of analyzing and mapping sites of lower optical reflectivity found on OCT, by examining eyes with various types of retinal edema to identify abnormal increases in retinal extracellular fluid.
Prospective analysis of a cohort of cases.
Healthy eyes and eyes with retinal edema in the setting of different retinal diseases.
Prospective OCT-Leakage analysis of 12 eyes with various types of retinal edema, such as diabetic macular edema, branch retinal vein occlusion, idiopathic perifoveal telangiectasia, and Irvine-Gass syndrome, representing intraretinal edema and eyes with idiopathic central serous chorioretinopathy and neovascular age-related macular degeneration representing subretinal fluid accumulation, in order to compare with OCT-Leakage analysis of a series of 41 eyes of 24 healthy controls. Raw scan data from the OCT images were exported and used to calculate lower than normal optical reflectivity maps (low optical reflectivity [LOR] ratios). Optical reflectivity LOR maps (OCT-Leakage maps) were collected for the full retina A-scan and layer by layer after segmentation. Low optical reflectivity ratios from patients with the different conditions of retinal edema and controls were compared. Fluorescein angiography (FA) and OCT angiography (OCTA) were performed in all eyes.
Identification of areas of abnormal retinal fluid accumulation.
The OCT-Leakage maps based on sites of LOR (LOR ratios) delineated the location of intraretinal and subretinal fluid, always integrating the location of the sites on FA and the vascular abnormalities observed on OCTA. The areas of fluid outline in the OCT-Leakage maps were coincident and generally larger than those seen on FA. In all cases, the OCT-Leakage maps were able to identify the location of the fluid in the different segmented retinal layers.
Mapping of lower reflectivity sites within the retina demonstrates the amount and location of retinal and subretinal fluid in different retinal diseases, showing potential to contribute to their management. Furthermore, the possibility of complementarity between OCT-Leakage and OCTA is highly promising.
通过检查患有各种类型视网膜水肿的眼睛，以识别视网膜细胞外液的异常增加，来测试OCT渗漏分析（OCT-Leakage），这是一种分析和绘制在光学相干断层扫描（OCT）上发现的低光学反射率部位的新方法。
对一组病例进行前瞻性分析。
健康眼睛以及患有不同视网膜疾病并伴有视网膜水肿的眼睛。
对12只患有各种类型视网膜水肿的眼睛进行前瞻性OCT渗漏分析，这些水肿类型包括糖尿病性黄斑水肿、视网膜分支静脉阻塞、特发性中心凹周围毛细血管扩张症和 Irvine-Gass综合征（代表视网膜内水肿），以及患有特发性中心性浆液性脉络膜视网膜病变和新生血管性年龄相关性黄斑变性（代表视网膜下液体积聚）的眼睛，以便与24名健康对照者的41只眼睛的OCT渗漏分析进行比较。从OCT图像中导出原始扫描数据，并用于计算低于正常光学反射率图（低光学反射率[LOR]比率）。在对整个视网膜进行A扫描并在分割后逐层收集光学反射率LOR图（OCT渗漏图）。比较不同视网膜水肿情况的患者与对照者的低光学反射率比率。所有眼睛均进行荧光素血管造影（FA）和OCT血管造影（OCTA）。
识别视网膜液异常积聚的区域。
基于LOR部位（LOR比率）的OCT渗漏图描绘了视网膜内和视网膜下液的位置，始终整合了FA上部位的位置以及OCTA上观察到的血管异常情况。OCT渗漏图中液体轮廓的区域与FA上的区域一致，且通常比FA上的区域大。在所有病例中，OCT渗漏图都能够识别不同分割视网膜层中液体的位置。
绘制视网膜内较低反射率部位的图谱可显示不同视网膜疾病中视网膜和视网膜下液的量和位置，显示出有助于疾病管理的潜力。此外，OCT渗漏分析和OCTA之间具有互补性的可能性非常有前景。
