Organ Transplant Centre, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Organ Donation and Transplant, Immunology, Guangzhou, China.
Artif Organs. 2023 Nov;47(11):1732-1741. doi: 10.1111/aor.14626. Epub 2023 Aug 8.
Normothermic machine perfusion (NMP) provides a novel platform to preserve isolated organs in an artificial condition. Our study aimed to explore the interaction between the liver and kidney at an ex vivo organ level by adding a liver to the kidney NMP circuit.
Porcine kidney and liver obtained from abattoir were subjected to 9 h NMP after suffering 30-min warm ischemia time and 90-min cold ischemia time. The liver-kidney NMP group (n = 5) and the single-kidney NMP group (n = 5) were designed. During the NMP, perfusion parameters, blood gas analysis, and tissue samples were compared.
The perfusate of both groups remained stable, and continuous urine production was observed during NMP. In the liver-kidney NMP group, the lactate level was low, while blood urea nitrogen increased and glucose levels decreased. After the NMP, the renal tissue in the liver-kidney group exhibited fewer histological changes such as tubular epithelium vacuolization, along with reduced expression of IL-6, IL-8, IL-1β, NLRP3, and GSDMD.
Our results indicated that the expression of renal pro-inflammatory factors was reduced in the liver-kidney NMP system.
常温机器灌注（NMP）为在人工条件下保存离体器官提供了一个新的平台。我们的研究旨在通过在肾脏 NMP 回路中添加肝脏来探索离体器官水平上肝脏和肾脏之间的相互作用。
从屠宰场获得的猪肾和肝在经历 30 分钟热缺血时间和 90 分钟冷缺血时间后进行 9 小时的 NMP。设计了肝-肾 NMP 组（n=5）和单肾 NMP 组（n=5）。在 NMP 期间，比较了灌注参数、血气分析和组织样本。
两组的灌流液均保持稳定，在 NMP 期间持续产生尿液。在肝-肾 NMP 组中，乳酸水平较低，而血尿素氮增加，葡萄糖水平降低。NMP 后，肝-肾组的肾组织中肾小管上皮细胞空泡化等组织学变化较少，IL-6、IL-8、IL-1β、NLRP3 和 GSDMD 的表达减少。
我们的结果表明，在肝-肾 NMP 系统中，肾前炎症因子的表达减少。
