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Combining independent protein and cellular SELEX with bioinformatic analysis may allow high affinity aptamer hit discovery.

作者信息

Ducrot Coline, Piffoux Max

机构信息

Pediatric Orthopedic Surgery Unit, Hôpital Femme Mère Enfant, Hospices civils de Lyon, Bron, France.

Team Cell Death and Pediatric Cancer, Cancer Initiation and Tumor Cell Identity Department, INSERM1052, CNRS5286, Cancer Research Center of Lyon, Lyon, France.

出版信息

Mol Ther Nucleic Acids. 2023 Jul 18;33:254-256. doi: 10.1016/j.omtn.2023.06.011. eCollection 2023 Sep 12.

DOI:10.1016/j.omtn.2023.06.011
PMID:37554514
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10404489/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f261/10404489/9837b2f57698/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f261/10404489/9837b2f57698/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f261/10404489/9837b2f57698/gr1.jpg

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本文引用的文献

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Combination of protein and cell internalization SELEX identifies a potential RNA therapeutic and delivery platform to treat EphA2-expressing tumors.蛋白质与细胞内化SELEX相结合,鉴定出一种潜在的用于治疗表达EphA2的肿瘤的RNA治疗和递送平台。
Mol Ther Nucleic Acids. 2023 May 8;32:758-772. doi: 10.1016/j.omtn.2023.05.003. eCollection 2023 Jun 13.
2
Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity.用于筛选具有细胞内化活性的人工核酸适配体的杂交型SELEX
Pharmaceutics. 2021 Jun 15;13(6):888. doi: 10.3390/pharmaceutics13060888.
3
Targeting EphA2 in cancer.
AptamerRunner: An accessible aptamer structure prediction and clustering algorithm for visualization of selected aptamers.
适体运行器:一种用于所选适体可视化的可访问的适体结构预测与聚类算法。
bioRxiv. 2023 Nov 15:2023.11.13.566453. doi: 10.1101/2023.11.13.566453.
靶向 EphA2 治疗癌症。
J Hematol Oncol. 2020 Aug 18;13(1):114. doi: 10.1186/s13045-020-00944-9.
4
Ligand-Guided Selection of Target-Specific Aptamers: A Screening Technology for Identifying Specific Aptamers Against Cell-Surface Proteins.配体引导的靶向特异性适配体筛选:一种用于鉴定针对细胞表面蛋白的特异性适配体的筛选技术。
Nucleic Acid Ther. 2016 Jun;26(3):190-8. doi: 10.1089/nat.2016.0611. Epub 2016 May 5.
5
Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors.适配体在肿瘤穿透方面优于抗体:基于适配体的诊疗方法在实体瘤中的应用
Theranostics. 2015 Jul 2;5(10):1083-97. doi: 10.7150/thno.11711. eCollection 2015.
6
Pegaptanib in the treatment of wet, age-related macular degeneration.培加他汀治疗湿性年龄相关性黄斑变性
Int J Nanomedicine. 2006;1(3):263-8.
7
Generation of species cross-reactive aptamers using "toggle" SELEX.使用“切换”SELEX技术生成物种交叉反应性适配体。
Mol Ther. 2001 Dec;4(6):567-73. doi: 10.1006/mthe.2001.0495.
8
High affinity ligands from in vitro selection: complex targets.体外筛选得到的高亲和力配体:复杂靶点
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2902-7. doi: 10.1073/pnas.95.6.2902.
9
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.指数富集的配体系统进化:噬菌体T4 DNA聚合酶的RNA配体
Science. 1990 Aug 3;249(4968):505-10. doi: 10.1126/science.2200121.