Li Jun, Guo Xianguang
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, China.
Mitochondrial DNA B Resour. 2023 Mar 13;8(3):398-401. doi: 10.1080/23802359.2023.2186722. eCollection 2023.
A complete mitogenome of the Przewalski's Wonder Gecko () from the Junggar Basin in Northwest China was determined by using polymerase chain reaction and directly sequenced with the primer walking method. The total length was 17,184 bp, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (CR). The order and structure of the genes were identical to those of congeners. The 13 PCGs contain four start codons (ATG, GTG, ATA, and ATC), three complete stop codons (TAA, TAG, and AGG), and two incomplete stop codons (T-, TA-). The concatenated PCGs were used to perform Bayesian phylogenetic analyses together with mitogenome data of the family Sphaerodactylidae and related representative taxa available in GenBank. The resulting tree recovered the monophyly of Sphaerodactylidae, and confirmed the sister relationship between and with strong support. The newly determined mitogenome will provide fundamental data for understanding the population genetic structure of in particular, and the mitochondrial DNA evolution in Sphaerodactylidae in general.
通过聚合酶链反应，利用引物步移法直接测序，测定了来自中国西北准噶尔盆地的普氏奇壁虎（）的完整线粒体基因组。其全长为17,184 bp，包含13个蛋白质编码基因（PCGs）、22个转运RNA基因（tRNAs）、两个核糖体RNA基因（rRNAs）和一个控制区（CR）。这些基因的顺序和结构与同属物种相同。13个PCGs包含四个起始密码子（ATG、GTG、ATA和ATC）、三个完整的终止密码子（TAA、TAG和AGG）以及两个不完整的终止密码子（T-、TA-）。串联的PCGs与球趾虎科的线粒体基因组数据以及GenBank中可用的相关代表性分类群一起用于进行贝叶斯系统发育分析。所得树状图恢复了球趾虎科的单系性，并以强有力的支持证实了与 之间的姐妹关系。新测定的线粒体基因组将为特别是了解 的种群遗传结构，以及一般了解球趾虎科的线粒体DNA进化提供基础数据。
