Molecular characteristics of gastric cancer with amplification.

Gastric cancer is a prevalent malignancy with a high degree of heterogeneity, which has led to a poor therapeutic response. Though there are numerous HER2-targeted medicines for HER2+ gastric cancer, many trials have not indicated an improvement in overall survival. Here 29 amplification (-Amp) type gastric cancer samples with WES and RNA-seq data were selected for investigation, which copy-number aberration (CNA) was +2. Initially, the somatic mutation and copy number variant (CNV) of them, which might cause resistance to HER2-targeted therapies, were systematically investigated evaluated, as well as their mutation signatures. Moreover, 37 modules were identified using weighted gene co-expression network analysis (WGCNA), including the blue module related to DFS status and lightcyan module correlated with __ rearrangement. In addition, focal adhesion and ECM-receptor interaction pathways were considerably enriched in the turquoise module with gene. ExportNetworkToCytoscape determined that and are tightly connected to ., Finally, 14 single-cell intestinal gastric cancer samples were investigated, and it was shown that the transcription factor regulon was highly expressed in group, as was the EMT score. Overall, our data provide comprehensive molecular characteristics of -Amp type gastric cancer, which offers additional information to improve HER2-targeted gastric cancer treatment.