微小RNA hsa-miR-425-5p和hsa-miR-4523的表达作为活动性肺结核、潜伏性结核感染和淋巴结结核的生物标志物。
microRNA hsa-miR-425-5p and hsa-miR-4523 expressions as biomarkers of active pulmonary tuberculosis, latent tuberculosis infection, and lymph node tuberculosis.
作者信息
Massi Muhammad Nasrum, Hidayah Najdah, Handayani Irda, Iskandar Israini Wiyulanda, Djannah Fathul, Angria Nirmawati, Halik Handayani
机构信息
Department of Clinical Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, 90245, South Sulawesi, Indonesia.
Hasanuddin University Medical Research Center Laboratory, Faculty of Medicine, Universitas Hasanuddin, Makassar, 90245, South Sulawesi, Indonesia.
出版信息
Noncoding RNA Res. 2023 Jul 21;8(4):527-533. doi: 10.1016/j.ncrna.2023.07.001. eCollection 2023 Dec.
BACKGROUND
Studies on miRNA highlight its significance as an immunomarker for several diseases, including tuberculosis. This study aimed to determine the difference between miR-425-5p and miR-4523 expressions in patients with active pulmonary TB (PTB), latent TB infection (LTBI), and lymph node TB (LNTB), whose diagnosis remains challenging.
METHODS
This case-control study was performed on blood samples obtained from 23 patients with PTB, 21 with LTBI, 21 with LNTB, and 25 healthy controls (HC). miRNA hsa-miR-425-5p and hsa-miR-4523 expression levels were measured by RT-qPCR. Statistical analyses were performed using SPSS version 25.0.
RESULTS
RT-qPCR showed that hsa-mir-425-5p and hsa-mir-4523 expression levels were significantly different among the four groups (PTB, LTBI, LNTB, and HCs). The hsa-mir-425-5p miRNA expression level in LNTB was higher than that in LTBI (p = 0.003). Meanwhile, the hsa-mir-4523 miRNA expression was downregulated in PTB and LNTB than in LTBI (p < 0.0001 and p = 0.015, respectively). The ROC analysis of a single sample showed that only mir-4523 could discriminate LTBI and HCs, with an AUC of 0.829 (p < 0.001). The ROC curve of each miRNA was further analyzed after logistic regression by adjusting for sex and age. The combination of both miRNAs was also analyzed. The model that analyzed the combination of both miRNAs after adjusting for age had the best performance in differentiating LNTB from LTBI, with an AUC of 0.97 (p < 0.001)
CONCLUSION
miRNA hsa-mir-425-5p was upregulated and miRNA hsa-mir-4523 was downregulated in PTB and LNTB than in LTBI.
背景
对微小RNA（miRNA）的研究突显了其作为包括结核病在内的多种疾病免疫标志物的重要性。本研究旨在确定活动性肺结核（PTB）、潜伏性结核感染（LTBI）和淋巴结结核（LNTB）患者中miR-425-5p和miR-4523表达的差异，这些疾病的诊断仍然具有挑战性。
方法
本病例对照研究对从23例PTB患者、21例LTBI患者、21例LNTB患者和25名健康对照（HC）获取的血样进行。通过逆转录定量聚合酶链反应（RT-qPCR）测量miRNA hsa-miR-425-5p和hsa-miR-4523的表达水平。使用SPSS 25.0版进行统计分析。
结果
RT-qPCR显示，hsa-mir-425-5p和hsa-mir-4523的表达水平在四组（PTB、LTBI、LNTB和HCs）之间存在显著差异。LNTB中hsa-mir-425-5p miRNA表达水平高于LTBI（p = 0.003）。同时，PTB和LNTB中hsa-mir-4523 miRNA表达低于LTBI（分别为p < 0.0001和p = 0.015）。单个样本的ROC分析显示，只有mir-4523能够区分LTBI和HCs，曲线下面积（AUC）为0.829（p < 0.001）。在对性别和年龄进行逻辑回归调整后，进一步分析了每个miRNA的ROC曲线。还分析了两种miRNA的组合。在调整年龄后分析两种miRNA组合的模型在区分LNTB和LTBI方面表现最佳，AUC为0.97（p < 0.001）。
结论
与LTBI相比，PTB和LNTB中miRNA hsa-mir-425-5p上调，miRNA hsa-mir-4523下调。
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