Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, P. R. China.
Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, P. R. China.
Anal Chem. 2023 Aug 22;95(33):12478-12486. doi: 10.1021/acs.analchem.3c02134. Epub 2023 Aug 9.
Cysteine (Cys) is a crucial biological thiol that has a vital function in preserving redox homeostasis in organisms. Studies have shown that Cys is closely related to the development of cancer. Thus, it is necessary to design an efficient method to detect Cys for an effective cancer diagnosis. In this work, a novel tumor-targeting probe (Bio-Cy-S) for dual-modal (NIR fluorescence and photoacoustic) Cys detection is designed. The probe exhibits high selectivity and sensitivity toward Cys. After reaction with Cys, both NIR fluorescence and photoacoustic signals are activated. Bio-Cy-S has been applied for the dual-modal detection of Cys levels in living cells, and it can be used to distinguish normal cells from cancer cells by different Cys levels. In addition, the probe is capable of facilitating dual-modal imaging for monitoring changes in Cys levels in tumor-bearing mice. More importantly, the excellent tumor-targeting ability of the probe greatly improves the signal-to-noise ratio of imaging. To the best of our knowledge, this is the first Cys probe to combine targeting and dual-modal imaging performance for cancer diagnosis.
半胱氨酸（Cys）是一种重要的生物巯基，对维持生物体的氧化还原平衡具有重要作用。研究表明，Cys 与癌症的发生发展密切相关。因此，设计一种有效的 Cys 检测方法对于癌症的有效诊断是非常必要的。本工作设计了一种新型的用于双模式（近红外荧光和光声）Cys 检测的肿瘤靶向探针（Bio-Cy-S）。该探针对 Cys 具有高选择性和高灵敏度。与 Cys 反应后，近红外荧光和光声信号均被激活。Bio-Cy-S 已被用于活细胞中 Cys 水平的双模式检测，并且可以通过不同的 Cys 水平区分正常细胞和癌细胞。此外，该探针还能够促进肿瘤荷瘤小鼠中 Cys 水平变化的双模式成像。更重要的是，探针优异的肿瘤靶向能力极大地提高了成像的信噪比。据我们所知，这是第一个将靶向性和双模式成像性能相结合用于癌症诊断的 Cys 探针。
