Guangdong Provincial Key Laboratory of New Drug Screening and Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Department of Musculoskeletal Oncology, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510642, China.
Anal Chem. 2022 Aug 2;94(30):10737-10744. doi: 10.1021/acs.analchem.2c01372. Epub 2022 Jul 23.
Cysteine (Cys) plays an important role in many physiological activities of human beings. Various diseases are always accompanied by abnormal levels of Cys. A series of Cys-responsive probes were recently developed. However, most fluorescent probes have many disadvantages and exhibit poor imaging. Therefore, a near-infrared fluorescence (NIRF)/photoacoustic (PA) dual-mode probe with high selectivity and sensitivity (limit of detection = 10.6 nM) toward Cys was developed in this study. The new Probe I interacted with Cys to activate NIRF/PA signals, detecting Cys with a large emission wavelength (851 nm) and Stokes shift (191 nm), monitoring the occurrence of liver cancer . This work not only presented an effective NIRF/PA dual-mode dicyanoisophorone probe for the first time in the imaging of Cys but also provided a comprehensive and accurate tool for detecting different analytes and tumors in deeper tissues, which could be conducive to the early diagnosis of diseases.
半胱氨酸（Cys）在人类的许多生理活动中起着重要作用。各种疾病总是伴随着 Cys 水平的异常。最近开发了一系列 Cys 响应探针。然而，大多数荧光探针存在许多缺点，表现出较差的成像性能。因此，本研究开发了一种对 Cys 具有高选择性和灵敏度（检测限=10.6 nM）的近红外荧光（NIRF）/光声（PA）双模探针。新型探针 I 与 Cys 相互作用以激活 NIRF/PA 信号，检测 Cys 具有较大的发射波长（851nm）和斯托克斯位移（191nm），监测肝癌的发生。这项工作不仅首次在 Cys 的成像中提出了一种有效的 NIRF/PA 双模二氰基异佛尔酮探针，而且为在更深组织中检测不同分析物和肿瘤提供了一种全面、准确的工具，这有助于疾病的早期诊断。
