Department of Biology, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2023 Oct;50(10):8163-8175. doi: 10.1007/s11033-023-08707-8. Epub 2023 Aug 9.
The presence of neural precursor stem cells (NPSCs) in some parts of the adult brain and the potency of these types of cells with a therapeutic viewpoint, has opened up a new approach for the treatment and recovery of the defects of central nervous system (CNS). Quercetin, as an herbal flavonoid, has been extensively investigated and shown to have numerous restoratives, inhibitory, and protective effects on some cell-lines and disorders. The purpose of this study is to simultaneously investigate the effect of quercetin on the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene and the effect on the proliferation and differentiation of NPSCs derived from the subventricular zone (SVZ) of the brain of adult rats.
The cell obtained from SVZ cultured for one week and treated with quercetin at the concentrations of 1, 5, and 15 μM to evaluate the Nrf2 expression, proliferation and differentiation of NSCs after one week. Cellular and genetic results was performed by RT-PCR, MTT assay test, quantification of images with Image-J and counting. The results indicated that the quercetin increases expression of Nrf2 at concentration above 5 μM. Also differentiation and proliferation rate of NSCs is affected by various concentrations of quercetin in a dose-dependent manner.
These findings confirmed the dose-dependent effect of quercetin on proliferation and differentiation of cell. In addition, quercetin increased the expression of Nrf2 gene. By combining these two effects of quercetin, this substance can be considered an effective compound in the treatment of degenerative defects in CNS.
成年大脑某些部位存在神经前体细胞（NPSCs），这些类型的细胞具有治疗潜力，为中枢神经系统（CNS）缺陷的治疗和恢复开辟了新途径。槲皮素作为一种草药类黄酮，已被广泛研究，对某些细胞系和疾病具有多种修复、抑制和保护作用。本研究旨在同时研究槲皮素对核因子红细胞 2 相关因子 2（Nrf2）基因表达的影响，以及对成年大鼠脑侧脑室下区（SVZ）来源的 NPSCs 增殖和分化的影响。
从 SVZ 获得的细胞培养一周，用 1、5 和 15 μM 的槲皮素处理，以评估 Nrf2 表达、一周后 NSCs 的增殖和分化。通过 RT-PCR、MTT 测定试验、Image-J 定量图像和计数进行细胞和遗传结果。结果表明，槲皮素在浓度高于 5 μM 时增加 Nrf2 的表达。此外，NSCs 的分化和增殖率也受到不同浓度槲皮素的浓度依赖性影响。
这些发现证实了槲皮素对细胞增殖和分化的剂量依赖性作用。此外，槲皮素增加了 Nrf2 基因的表达。通过结合槲皮素的这两种作用，这种物质可以被认为是治疗 CNS 退行性缺陷的有效化合物。
