Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials, Lanzhou, 730070, People's Republic of China.
Mikrochim Acta. 2023 Aug 9;190(9):346. doi: 10.1007/s00604-023-05866-0.
A novel electrochemical biosensor with excellent performance was fabricated for levofloxacin (LEV) detection, which adopted triangular Ag nanoplates (Tri-AgNP) confined in a poly-L-cysteine (poly-L-Cys) film and a semiconducting covalent organic framework (COF) as the electrochemical sensing material. The developed electrochemical sensor revealed excellent analytical properties because of its good electrical conductivity, fast electron transfer, and abundant bioactive site. Based on this, a linear relationship between the LEV concentration and the peak current response at 0.92 V was obtained under the optimal experimental conditions by differential pulse voltammetry (DPV), with a wide linear range of 0.05 to 600 μM and a low limit of detection (LOD) of 0.0061 μM. The prepared sensor also realized sensitive and accurate determination of LEV in human serum and urine samples by standard addition method, with satisfactory recoveries (97.1 to 104%) and a low relative standard deviation of less than 4.6%. These results indicated that the novel ternary system has a promising application in the development of electrochemical signal probe and electrochemical biosensing platform.
一种具有优异性能的新型电化学生物传感器被制备用于左氧氟沙星（LEV）的检测，该传感器采用了三角形银纳米片（Tri-AgNP）限制在聚-L-半胱氨酸（poly-L-Cys）薄膜和半导体共价有机框架（COF）中作为电化学传感材料。由于其良好的导电性、快速的电子转移和丰富的生物活性位点，所开发的电化学传感器显示出优异的分析性能。在此基础上，通过差分脉冲伏安法（DPV）在最佳实验条件下，在 0.92 V 处的峰电流响应与 LEV 浓度之间获得了线性关系，线性范围为 0.05 至 600 μM，检测限（LOD）低至 0.0061 μM。通过标准加入法，该制备的传感器还实现了对人血清和尿液样本中 LEV 的灵敏和准确测定，回收率令人满意（97.1%至 104%），相对标准偏差低于 4.6%。这些结果表明，新型三元体系在电化学信号探针和电化学生物传感平台的开发方面具有广阔的应用前景。
