Kimura N, Toyonaga B, Yoshikai Y, Triebel F, Debre P, Minden M D, Mak T W
J Exp Med. 1986 Sep 1;164(3):739-50. doi: 10.1084/jem.164.3.739.
The nucleotide sequences of 22 human T cell antigen receptor (TcR) beta chain variable region genes isolated from various T lymphocytes have been analyzed. Of the 19 variable gene segment (V beta)-containing sequences, 17 were unique. The V beta gene segments were grouped into 11 families. Comparisons were made with the data of Concannon et al. to unify the nomenclature. The data is consistent with a total V beta gene segment repertoire with a most probable value of 38 members and an upper bound of 104 members at the 95% confidence level. Southern blot data of germline DNA using selected TcR V beta cDNAs as probes support this estimate. The human repertoire is approximately three to four times greater than that reported for the mouse. Explanations for this discrepancy are proposed.
对从各种T淋巴细胞中分离出的22个人类T细胞抗原受体(TcR)β链可变区基因的核苷酸序列进行了分析。在19个含有可变基因片段(Vβ)的序列中,17个是独特的。Vβ基因片段被分为11个家族。与康坎农等人的数据进行了比较,以统一命名法。数据与一个总Vβ基因片段库一致,在95%置信水平下,最可能的值为38个成员,上限为104个成员。使用选定的TcR Vβ cDNA作为探针的种系DNA的Southern印迹数据支持这一估计。人类的基因库大约是小鼠报道的基因库的三到四倍。对这种差异提出了解释。