Ahmed Salahuddin, Keniry Megan, Padilla Victoria, Anaya-Barbosa Narcedalia, Javed Md Noushad, Gilkerson Robert, Gomez Kithzia, Ashraf Ali, Narula Acharan S, Lozano Karen
Department of Mechanical Engineering, The University of Texas Rio Grande Valley, Edinburg, TX, USA.
Department of Biology, The University of Texas Rio Grande Valley, Edinburg, TX, USA.
Int J Biol Macromol. 2023 Oct 1;250:126187. doi: 10.1016/j.ijbiomac.2023.126187. Epub 2023 Aug 8.
This study investigates the feasibility of centrifugal spinning for producing fibrous membranes containing pullulan, chitosan, and danshen extract. The danshen extract is composed of 20 wt% salvianolic acid B (SA). Citric acid was added to the mixture as a crosslinking agent to promote its use in the aqueous medium. The influence of the danshen concentration (25 wt% and 33 wt%) on fiber morphology, thermal behavior, and the biochemical effect was analyzed. Developed fiber-based membranes consist of long, continuous, and uniform fibers with a sparse scattering of beads. Fiber diameter analysis shows values ranging from 384 ± 123 nm to 644 ± 141 nm depending on the concentration of danshen. The nanofibers show adequate aqueous stability after crosslinking. Thermal analysis results prove that SA is loaded into nanofibers without compromising their structural integrity. Cell-based results indicate that the developed nanofiber membranes promote cell growth and are not detrimental to fibroblast cells. Anticancer studies reveal a promising inhibition to the proliferation of HCT116 colon cancer cells. The developed systems show potential as innovative systems to be used as a bioactive chemotherapeutic drug that could be placed on the removed tumor site to prevent development of colon cancer microdeposits.
本研究探讨了离心纺丝法制备含普鲁兰多糖、壳聚糖和丹参提取物的纤维膜的可行性。丹参提取物由20 wt%的丹酚酸B（SA）组成。向混合物中加入柠檬酸作为交联剂，以促进其在水性介质中的应用。分析了丹参浓度（25 wt%和33 wt%）对纤维形态、热行为和生化效应的影响。所制备的纤维基膜由长而连续且均匀的纤维组成，珠子稀疏分布。纤维直径分析表明，根据丹参浓度不同，其值在384±123 nm至644±141 nm之间。交联后，纳米纤维表现出足够的水稳定性。热分析结果证明，SA被负载到纳米纤维中，而不损害其结构完整性。基于细胞的结果表明，所制备的纳米纤维膜促进细胞生长，对成纤维细胞无害。抗癌研究显示对HCT116结肠癌细胞的增殖有良好的抑制作用。所开发的系统显示出作为创新系统的潜力，可作为一种生物活性化疗药物放置在切除的肿瘤部位，以预防结肠癌微沉积物的形成。
