Hughes Peyton, Marshall Damian, Reid Yvonne, Parkes Helen, Gelber Cohava
ATCC, Manassas, VA 20110, USA.
Biotechniques. 2007 Nov;43(5):575, 577-8, 581-2 passim. doi: 10.2144/000112598.
Increasing data demonstrate that cellular cross-contamination, misidentified cell lines, and the use of cultures at high-passage levels contribute to the generation of erroneous and misleading results as well as wasted research funds. Contamination of cell lines by other lines has been recognized and documented back to the 1950s. Based on submissions to major cell repositories in the last decade, it is estimated that between 18% and 36% of cell lines may be contaminated or misidentified. More recently, problems surrounding practices of over-subculturing cells are being identified. As a result of selective pressures and genetic drift, cell lines, when kept in culture too long, exhibit reduced or altered key functions and often no longer represent reliable models of their original source material. A review of papers showing significant experimental variances between low- and high-passage cell culture numbers, as well as contaminated lines, makes a strong case for using verified, tested cell lines at low- or defined passage numbers. In the absence of cell culture guidelines, mandates from the National Institutes of Health (NIH) and other funding agencies or journal requirements, it becomes the responsibility of the scientific community to perform due diligence to ensure the integrity of cell cultures used in research.
越来越多的数据表明，细胞交叉污染、细胞系错误鉴定以及使用高传代水平的培养物会导致产生错误和误导性的结果，以及造成研究资金的浪费。细胞系被其他细胞系污染的情况早在20世纪50年代就已被认识到并记录在案。根据过去十年向主要细胞库提交的数据估计，18%至36%的细胞系可能被污染或错误鉴定。最近，围绕过度传代培养细胞的做法所产生的问题也逐渐被发现。由于选择压力和基因漂移，细胞系在培养时间过长时，其关键功能会降低或改变，并且往往不再代表其原始来源材料的可靠模型。一项对显示低传代和高传代细胞培养数量之间以及污染细胞系之间存在显著实验差异的论文的综述，有力地证明了应使用经过验证、检测且传代次数低或确定的细胞系。在缺乏细胞培养指南、美国国立卫生研究院（NIH）及其他资助机构的指令或期刊要求的情况下，科学界有责任进行尽职调查，以确保研究中使用的细胞培养物的完整性。
