在造釉细胞瘤中干细胞标志物 SALL4、LIN28A 和 KLF4 的表达。
Expression of stem cell markers SALL4, LIN28A, and KLF4 in ameloblastoma.
机构信息
Laboratory of Pathological Anatomy and Immunohistochemistry, Federal University of Pará, Belém, Pará, Brazil.
Cell Cultivation Laboratory, Federal University of Pará, Belém, Pará, Brazil.
出版信息
Diagn Pathol. 2023 Aug 9;18(1):92. doi: 10.1186/s13000-023-01379-9.
BACKGROUND
Ameloblastoma (AME) is a benign odontogenic tumour of epithelial origin characterised by slow but aggressive growth, infiltration, and recurrence; it is capable of reaching large dimensions and invading adjacent structures. Stem cell research has proven to be significant in the sphere of tumour biology through these cells' possible involvement in the aetiopathogenesis of this tumour.
METHODS
Immunohistochemistry was performed on AME, dentigerous cyst (DC), and dental follicle (DF) samples, and indirect immunofluorescence was performed on the AME-hTERT cell line to determine the expression of SALL4, LIN28A, and KLF4.
RESULTS
Expression of proteins related to cellular pluripotency was higher in AME cells than in DC and DF cells. The analysis revealed that the proteins in question were mainly expressed in the parenchyma of AME tissue samples and were detected in the nuclei of AME-hTERT cells.
CONCLUSIONS
Stem cells may be related to the origin and progression of AME.
背景
造釉细胞瘤（AME）是一种良性牙源性上皮来源的肿瘤，其生长缓慢但具有侵袭性、浸润性和复发性，能够达到较大的尺寸并侵犯相邻结构。通过这些细胞可能参与肿瘤发生，干细胞研究已被证明在肿瘤生物学领域具有重要意义。
方法
对造釉细胞瘤、牙囊（DC）和牙滤泡（DF）样本进行免疫组织化学染色，对造釉细胞瘤-hTERT 细胞系进行间接免疫荧光染色，以确定 SALL4、LIN28A 和 KLF4 的表达。
结果
与细胞多能性相关的蛋白在 AME 细胞中的表达高于 DC 和 DF 细胞。分析表明，这些蛋白主要在 AME 组织样本的实质中表达，并在 AME-hTERT 细胞的核中检测到。
结论
干细胞可能与 AME 的起源和进展有关。
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