Fang Ruiqiu, Chen Xiaolong, Shen Jie, Wang Bin
Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, 322100, Zhejiang, China.
Department of Life Sciences, Changzhi University, Changzhi, 046011, Shanxi, China.
Plant Methods. 2023 Aug 9;19(1):81. doi: 10.1186/s13007-023-01053-7.
N6-methyladenosine (m6A) is an important epigenetic modification involved in RNA stability and translation regulation. Manipulating the expression of RNA m6A methyltransferases or demethylases makes it difficult to study the effect of specific RNA methylation.
In this study, we report the development of Plant m6A Editors (PMEs) using dLwaCas13a (from L. wadei) and human m6A demethylase ALKBH5 catalytic domain. PMEs specifically demethylates m6A of targeted mRNAs (WUS, STM, FT, SPL3 and SPL9) to increase mRNAs stability. In addition, we discovered that a double ribozyme system can significantly improve the efficiency of RNA editing.
PMEs specifically demethylates m6A of targeted mRNAs to increase mRNAs stability, suggesting that this engineered tool is instrumental for biotechnological applications.
N6-甲基腺苷（m6A）是一种重要的表观遗传修饰，参与RNA稳定性和翻译调控。操纵RNA m6A甲基转移酶或去甲基酶的表达使得研究特定RNA甲基化的作用变得困难。
在本研究中，我们报道了利用dLwaCas13a（来自瓦氏李斯特菌）和人m6A去甲基酶ALKBH5催化结构域开发的植物m6A编辑器（PMEs）。PMEs特异性地使靶向mRNA（WUS、STM、FT、SPL3和SPL9）的m6A去甲基化，以提高mRNA的稳定性。此外，我们发现双核酶系统可以显著提高RNA编辑效率。
PMEs特异性地使靶向mRNA的m6A去甲基化以提高mRNA的稳定性，表明这种工程工具对生物技术应用具有重要作用。
