Analysis of the role of glucose metabolism-related genes in dilated cardiomyopathy based on bioinformatics.

BACKGROUND

Dilated cardiomyopathy (DCM) is a prevalent condition with diverse etiologies, including viral infection, autoimmune response, and genetic factors. Despite the crucial role of energy metabolism in cardiac function, therapeutic targets for key genes in DCM's energy metabolism remain scarce.

METHODS

Our study employed the GSE79962 and GSE42955 datasets from the Gene Expression Omnibus (GEO) database for myocardial tissue sample collection and target gene identification via differential gene expression screening. Using various R packages, GSEA software, and the STRING database, we conducted data analysis, gene set enrichment, and protein-protein interaction predictions. The least absolute shrinkage and selection operator (LASSO) and Support Vector Machine (SVM) algorithms aided in feature gene selection, while the predictive model's efficiency was evaluated via the receiver operating characteristic (ROC) curve analysis. We used the non-negative matrix factorization (NMF) method for molecular typing and the cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm for predicting immune cell infiltration.

RESULTS

The and genes may regulate the immune microenvironment of DCM by influencing activated dendritic cells, activated mast cells, and M0 macrophages, respectively. The , and genes may regulate m6A methylation in DCM by affecting the , and genes. Further regulatory mechanism analysis suggested that , and could be involved in the development of cardiomyopathy by regulating the Toll-like receptor signaling pathway.

CONCLUSIONS

, and may serve as potential targets for guiding the diagnosis, treatment, and follow-up of DCM.