Wustrow T P, Raffael A, Valet G
Arch Otolaryngol Head Neck Surg. 1986 Oct;112(10):1078-84. doi: 10.1001/archotol.1986.03780100066010.
Individual chemosensitivity assessment of solid tumors has been hindered by the lack of a reliable and rapid test system for predicting inhibition of tumor cell growth in vitro of all malignant cells and for distinguishing between inflammatory and tumor cells. Six long-term cultured and recloned squamous cell carcinoma lines of the head and neck were analyzed using a newly developed multiparametric flow cytometric technique. After incubation with cytotoxic drugs at different time intervals, each cell line showed its own chemosensitive profile. With this multiparametric staining technique, it is possible to monitor the in vitro response of tumor cells and to compare their quantitative behavior with that of nonmalignant inflammatory cells in solid tumors of the head and neck.
由于缺乏一种可靠且快速的检测系统，用以预测体外对所有恶性细胞肿瘤细胞生长的抑制作用以及区分炎症细胞和肿瘤细胞，实体瘤的个体化学敏感性评估受到了阻碍。使用一种新开发的多参数流式细胞术对六种长期培养和再克隆的头颈部鳞状细胞癌系进行了分析。在不同时间间隔与细胞毒性药物孵育后，每个细胞系都显示出其自身的化学敏感性特征。通过这种多参数染色技术，可以监测肿瘤细胞的体外反应，并将其定量行为与头颈部实体瘤中的非恶性炎症细胞进行比较。
