Garozzo A, Rossi M, Denaro A, Allegra E, Santangelo G, Amato M, Tomasello C
Istituto di Otorinolaringoiatria dell'Università di Catania, Italy.
J Chemother. 1989 Feb;1(1):59-63. doi: 10.1080/1120009x.1989.11738865.
A new method to test the sensitivity of human tumor cells has been developed. A suspension of mechanically dissociated tumor cells is kept in continuous incubation for 24h, in cultures with antineoplastic agents. Drug induced cell cycle perturbations are monitored by flow cytometric computer analysis and DNA distributions of the cells stained with propidium iodide are expressed in percentage. The test is used in 15 head and neck human solid tumors. The drugs tested were: VCR, EpiDx, CDDP, MTX, 5-FU, CPM, BLM. The results obtained reveal that tumor sensitivity varies independently from the stage and malignity grading. Therapeutic combinations are assigned by selecting the drugs on the basis of the individual in vitro response.
已开发出一种检测人类肿瘤细胞敏感性的新方法。将机械解离的肿瘤细胞悬液在含有抗肿瘤药物的培养基中连续培养24小时。通过流式细胞仪计算机分析监测药物诱导的细胞周期扰动，并用碘化丙啶染色的细胞的DNA分布以百分比表示。该试验用于15例头颈部人类实体瘤。所测试的药物有：长春新碱、表柔比星、顺铂、甲氨蝶呤、5-氟尿嘧啶、环磷酰胺、博来霉素。所得结果表明，肿瘤敏感性与分期和恶性分级无关。根据个体体外反应选择药物来确定治疗组合。
