Kaplan A S, Caldarelli D D, Chacho M S, Bruce D R, Hutchinson J, Conway S, Coon J S
Arch Otolaryngol Head Neck Surg. 1986 Nov;112(11):1159-62. doi: 10.1001/archotol.1986.03780110035003.
Abnormal cellular DNA content, a hallmark of malignancy, is known to occur in squamous cell carcinoma of the head and neck. The significance of this finding has yet to be elucidated. Paraffin-embedded blocks from 46 squamous cell carcinomas of the head and neck were sectioned, dewaxed, and made into a suspension of bare nuclei by mechanical mincing and enzymatic digestion. The nuclei were stained with propidium iodide, and quantitative DNA measurements were obtained with flow cytometry. The results were compared with existing prognostic factors: DNA aneuploidy was identified in 41% of the specimens and was significantly correlated with tumor size and surgical stage; the relationship between DNA aneuploidy and anatomic site approaches statistical significance. There was no correlation with histologic grade or presence or absence of cervical metastasis.
异常细胞DNA含量是恶性肿瘤的一个标志，已知其在头颈部鳞状细胞癌中出现。这一发现的意义尚未阐明。对46例头颈部鳞状细胞癌的石蜡包埋块进行切片、脱蜡，并通过机械切碎和酶消化制成裸核悬液。细胞核用碘化丙啶染色，并用流式细胞术进行DNA定量测量。将结果与现有的预后因素进行比较：41%的标本中发现DNA非整倍体，且与肿瘤大小和手术分期显著相关；DNA非整倍体与解剖部位之间的关系接近统计学意义。与组织学分级或有无颈部转移无关。
