基于宏基因组测序的胎膜早破孕妇阴道微生物群与新生儿败血症之间的关联
Association between vaginal microbiomes and neonatal septicemia in pregnant women with preterm premature rupture of membranes based on metagenome sequencing.
作者信息
Yang Yong, Liu Zhaoming, Chen Xiaoyan, Qin Feng, Zhou Wei
机构信息
Department of Obstetrics and Gynecology, Chongqing Health Center for Women and Children, Women and Children's Hospital of Chongqing Medical University Chongqing, China.
出版信息
Am J Transl Res. 2023 Jul 15;15(7):4544-4557. eCollection 2023.
BACKGROUND
Preterm premature rupture of membranes (PPROM) is closely associated with pathogenic microbiomes in the female reproductive tract, and can lead to neonatal septicemia. The current study aimed to investigate potential pathogenic microbiomes associated with neonatal septicemia based on DNA metagenome sequencing.
METHODS
In this study, a total of 7 pregnant women with PPROM presenting neonatal septicemia (experimental group) and 3 pregnant women with normal newborns (control group) were enrolled. Vaginal secretions at admission and before parturition as well as placental tissues after parturition were collected for DNA metagenome sequencing using whole genome shotgun method on the Illumina NovaSeq/HiSeq platform. Raw data were processed by BioBakery workflow, and MetaPhlAn4 was implemented for qualitative and quantitative analyses of microbiome. Lactobacillus crispatus, Gardneralla vaginalis, Fannyhessea vaginae and Streptococcus suis were specifically detected from the experimental group. The two groups were compared using Student's t-tests.
RESULTS
The indexes of Chao1 (P=0.00028/P=0.00072), abundance-based coverage estimator (ACE, P=0.00059/P=0.00026), Shannon (P=0.036/P=0.0065) and Simpson (P=0.007/P=0.041) in the experimental group were increased at admission and before parturition as compared with the control group. Several microbiomes, such as Lactobacillus crispatus, Gardneralla vaginalis, Fannyhessea vaginae and Streptococcus suis, were specifically detected in the experimental group. Notably, Gardnerella vaginalis and Streptococcus gallolyticus were identified from the vaginal secretions and placenta tissues of women with neonatal septicemia. Moreover, nucleic acid synthesis and carbohydrate metabolism-related pathways were enriched in the experimental group.
CONCLUSION
This study enhanced the current understanding of the mechanisms underlying pathogenic microbiomes in PPROM-induced neonatal septicemia. The trial registry number is ChiCTR2300070666 (URL: https://www.chictr.org.cn/showproj.html?proj=195648).
背景
胎膜早破（PPROM）与女性生殖道中的致病微生物群密切相关，并可导致新生儿败血症。本研究旨在基于DNA宏基因组测序调查与新生儿败血症相关的潜在致病微生物群。
方法
本研究共纳入7例发生新生儿败血症的胎膜早破孕妇（实验组）和3例新生儿正常的孕妇（对照组）。在Illumina NovaSeq/HiSeq平台上，采用全基因组鸟枪法对入院时、分娩前的阴道分泌物以及分娩后的胎盘组织进行DNA宏基因组测序。原始数据通过BioBakery工作流程进行处理，并使用MetaPhlAn4对微生物群进行定性和定量分析。从实验组中特异性检测到卷曲乳杆菌、阴道加德纳菌、阴道芬内西酵母和猪链球菌。两组采用Student t检验进行比较。
结果
与对照组相比，实验组入院时和分娩前的Chao1指数（P=0.00028/P=0.00072）、基于丰度的覆盖率估计器（ACE，P=0.00059/P=0.00026）、香农指数（P=0.036/P=0.0065）和辛普森指数（P=0.007/P=0.041）均升高。在实验组中特异性检测到几种微生物群，如卷曲乳杆菌、阴道加德纳菌、阴道芬内西酵母和猪链球菌。值得注意的是，从发生新生儿败血症的女性的阴道分泌物和胎盘组织中鉴定出阴道加德纳菌和解脲链球菌。此外，实验组中核酸合成和碳水化合物代谢相关途径得到富集。
结论
本研究加深了对PPROM所致新生儿败血症中致病微生物群潜在机制的理解。试验注册号为ChiCTR2300070666（网址：https://www.chictr.org.cn/showproj.html?proj=195648）。
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