Vega-Polanco Mayumi, Solís-Montero Lislie, Rojas Julio C, Cruz-López Leopoldo, Alavez-Rosas David, Vallejo-Marín Mario
El Colegio de la Frontera Sur (ECOSUR), Unidad Tapachula, Carretera Antiguo Aeropuerto km 2.5, AP 36, Tapachula C.P. 30700, Chiapas, México.
Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT), Av. Insurgentes Sur 1582, Colonia Crédito Constructor, Delegación Benito Juárez, Ciudad de México C.P. 03940, México.
AoB Plants. 2023 Jul 21;15(4):plad049. doi: 10.1093/aobpla/plad049. eCollection 2023 Jul.
Floral scents shape plant-pollinator interactions. Although populations of the same species can vary in their floral scent, little is known about how this variation affects pollinator visitation. In this study, we compare the scents emitted by buzz-pollinated (Solanaceae) in two areas of its distribution (Mexico and USA) and investigate how these differences in scent affect pollinator preferences and attraction. We determined the variation of floral volatile compounds using hexane extraction followed by gas chromatography coupled with spectrometry. We also performed a field cage multiple-choice bioassay and a Y-tube behavioural bioassay using . We recorded 13 volatile compounds in floral extracts for plants from both ranges that varied qualitative and semi-quantitatively among populations. We found that in the field cage experiment, bumble bees visited plants from the US populations more frequently than plants from Mexican populations. However, bees showed no difference in preference between extracts from Mexican or US flowers. We conclude that although bees show differential visitation to whole plants of different regions, variation in floral extract alone does not translate into differences in preference by . The potential effects of variation in floral scent on the other native bee pollinators remain to be assessed.
花香塑造了植物与传粉者之间的相互作用。尽管同一物种的不同种群在花香方面可能存在差异，但对于这种差异如何影响传粉者的访花行为却知之甚少。在本研究中，我们比较了自花传粉植物（茄科）在其两个分布区域（墨西哥和美国）所散发的气味，并探究这些气味差异如何影响传粉者的偏好和吸引力。我们采用己烷萃取法，随后结合气相色谱-质谱联用技术来测定花香挥发性化合物的变化。我们还使用[具体实验对象未给出]进行了田间笼养多项选择生物测定和Y型管行为生物测定。我们记录了两个分布区域植物花香提取物中的13种挥发性化合物，这些化合物在不同种群之间存在定性和半定量的差异。我们发现在田间笼养实验中，大黄蜂访问美国种群植物的频率高于墨西哥种群的植物。然而，蜜蜂对墨西哥或美国花朵提取物的偏好没有差异。我们得出结论，尽管蜜蜂对不同区域的整株植物表现出不同的访花频率，但仅花香提取物的差异并不能转化为[具体实验对象未给出]偏好的差异。花香变化对其他本地蜜蜂传粉者的潜在影响仍有待评估。
