Clin Lab. 2023 Aug 1;69(8). doi: 10.7754/Clin.Lab.2023.230230.
This study aimed to demonstrate potential measures to discern interference and establish an algorithm for identifying interference in an hs-cTnT assay with a clinical discrepancy.
Laboratory error and disease factors were first excluded. Then, a serial dilution test, polyethylene glycol (PEG) precipitation, and heterophile antibody blocking reagents (HBRs) were performed to confirm the interference substance. In addition, high-speed centrifugation and western blot were tried.
The sample diluted linearly (R2 = 0.9966). However, there was a dramatic reduction in the concentration of hs-cTnT concentration to a normal level after both PEG precipitation and HBR1 treatment. The results were inconclusive in high-speed centrifugation and western blot assay.
This study elucidated a heterophile antibody interference in measurement of hs-cTnT alone on a Roche analyzer.
本研究旨在展示潜在的措施来辨别干扰，并建立一种算法，以识别 hs-cTnT 检测中与临床差异相关的干扰。
首先排除实验室误差和疾病因素。然后，进行一系列稀释试验、聚乙二醇（PEG）沉淀和异嗜性抗体阻断试剂（HBR）以确认干扰物质。此外，还尝试了高速离心和 Western blot。
样品呈线性稀释（R2 = 0.9966）。然而，PEG 沉淀和 HBR1 处理后，hs-cTnT 浓度显著降低至正常水平。高速离心和 Western blot 检测结果不确定。
本研究阐明了罗氏分析仪上 hs-cTnT 单独检测中存在异嗜性抗体干扰。
