Heart and Vascular Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
Clin Chim Acta. 2020 Jun;505:98-99. doi: 10.1016/j.cca.2020.02.006. Epub 2020 Feb 6.
The fifth generation (high-sensitivity) troponin T assay offers increased precision and analytical sensitivity to the predecessor method. The assay has proven utility in risk stratification and patient management. Upon clinical suspicion and discordant 4th generation troponin T and troponin I results, we investigated a sample for suspected interfering substances to the 5th generation troponin T assay.
The analysis included a serial dilution, treatment with polyethylene glycol, commercial antibody blocking reagents, and size exclusion chromatography.
The sample diluted linearly (R = 0.9957); however, experienced a dramatic reduction in concentration after both the polyethylene glycol and blocking agent treatment. Finally, size exclusion chromatography demonstrated assay reactivity around 970 kDa range.
These experiments elucidate a heterophilic antibody interference to the assay, and demonstrate potential measures to discern the interference.
第五代（高敏）肌钙蛋白 T 检测方法在前代方法的基础上提高了精度和分析灵敏度。该检测方法已被证明在风险分层和患者管理方面具有实际效用。在临床怀疑和第四代肌钙蛋白 T 和肌钙蛋白 I 检测结果不一致的情况下，我们调查了一个疑似对第五代肌钙蛋白 T 检测有干扰物质的样本。
分析包括连续稀释、聚乙二醇处理、商业抗体阻断试剂和分子筛层析。
样本呈线性稀释（R = 0.9957）；然而，在聚乙二醇和阻断剂处理后，浓度急剧下降。最后，分子筛层析显示检测反应在 970 kDa 左右的范围。
这些实验阐明了对检测的异嗜性抗体干扰，并展示了潜在的辨别干扰的措施。
