Naseer Nadia, Mustafa Muhammad Munam, Latief Noreen, Fazal Numan, Tariq Muhammad, Afreen Afshan, Yaqub Faiza, Riazuddin Sheikh
Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.
Department of Biotechnology, Mirpur University of Science and Technology (MUST), Mirpur, Pakistan.
J Biomed Mater Res B Appl Biomater. 2023 Dec;111(12):2032-2043. doi: 10.1002/jbm.b.35303. Epub 2023 Aug 10.
Oxidative stress is a destructive phenomenon that affects various cell structures including membranes, proteins, lipoproteins, lipids, and DNA. Oxidative stress and inflammation owing to lifestyle changes may lead to serious diseases such as Cancers, Gout, and Arthritis etc. These disorders can be prevented using different therapeutic strategies including nanomedicine. Biosynthesized gold nanoparticles (GNPs) because of their anti-inflammatory and antioxidant bioactivities can be key player in reversal of these ailments. This study was carried out to evaluate the anti-inflammatory and antioxidant potential of bio fabricated GNPs with Sarcococca saligna (S. saligna) extract on injured human adipose-derived Mesenchymal stem cells (hADMSCs). GNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and energy dispersive x-ray (EDS). Phytochemical screening of biosynthesized GNPs exhibited a significant release of polyphenols, that is, total phenolic content (TPC) and total flavonoid content (TFC). GNPs priming amended the in vitro injury caused by Monosodium Iodoacetate (MIA) as exhibited by improved cell viability, wound closure response and superoxide dismutase activity (SOD). The anti-inflammatory conduct assessed through NF-κB pathway and other associated inflammatory markers reported down-regulation of TNF-α (0.644 ± 0.045), IL-1β (0.694 ± 0.147) and IL-6 (0.622 ± 0.112), apoptosis causing genes like Caspase-3 (0.734 ± 0.13) and BAX (0.830 ± 0.12), NF-κB pathway, p65 (0.672 ± 0.084) and p105 (0.539 ± 0.083) associated genes. High SOD activity (95 ± 5.25%) revealed by treated hADMSCs with GNPs also supported the antioxidant role of GNPs in vitro model. This study concludes that S. saligna bio fabricated GNPs priming may improve the therapeutic potential of hADMSCs against chronic inflammatory problems by regulating NF-κB pathway.
氧化应激是一种破坏性现象，会影响包括细胞膜、蛋白质、脂蛋白、脂质和DNA在内的各种细胞结构。由于生活方式改变导致的氧化应激和炎症可能会引发严重疾病，如癌症、痛风和关节炎等。可以采用包括纳米医学在内的不同治疗策略来预防这些疾病。生物合成的金纳米颗粒（GNPs）因其抗炎和抗氧化生物活性，可能成为逆转这些疾病的关键因素。本研究旨在评估用柳叶腊梅（S. saligna）提取物生物制备的GNPs对受损的人脂肪来源间充质干细胞（hADMSCs）的抗炎和抗氧化潜力。通过紫外可见（UV-Vis）光谱、扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和能量色散X射线（EDS）对GNPs进行了表征。对生物合成的GNPs进行植物化学筛选，结果显示多酚有显著释放，即总酚含量（TPC）和总黄酮含量（TFC）。GNPs预处理改善了碘乙酸钠（MIA）引起的体外损伤，表现为细胞活力提高、伤口闭合反应增强和超氧化物歧化酶活性（SOD）增加。通过NF-κB途径和其他相关炎症标志物评估的抗炎行为表明，肿瘤坏死因子-α（TNF-α）（0.644±0.045）、白细胞介素-1β（IL-1β）（0.694±0.147）和白细胞介素-6（IL-6）（0.622±0.112）、凋亡诱导基因如半胱天冬酶-3（Caspase-3）（0.734±0.13）和BAX（0.830±0.12）、NF-κB途径、p65（0.672±0.084）和p105（0.539±0.083）相关基因均下调。用GNPs处理的hADMSCs显示出高SOD活性（95±5.25%），这也支持了GNPs在体外模型中的抗氧化作用。本研究得出结论，柳叶腊梅生物制备的GNPs预处理可能通过调节NF-κB途径提高hADMSCs对慢性炎症问题的治疗潜力。
