National Centre of Excellence in Molecular Biology, University of the Punjab , Lahore, Pakistan.
Allama Iqbal Medical College, University of Health Sciences , Lahore, Pakistan.
Adipocyte. 2020 Dec;9(1):495-508. doi: 10.1080/21623945.2020.1812242.
Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC's transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC's and effect of on stressed hADMSCs. Results of these studies provided evidence that protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.
间充质干细胞（MSCs）因其具有很强的再生能力而被用于治疗许多疾病。然而，间充质干细胞的移植有时并不能产生最大的再生效果，因为它们无法在炎症环境中存活。包括预处理在内的几种方法被用于提高间充质干细胞的存活率。最近报道的一种方法是用植物提取物预处理间充质干细胞。本研究旨在评估 提取物对应激人脂肪间充质干细胞（hADMSCs）的影响。分离的 hADMSCs 用不同浓度的 提取物预处理，通过各种测定和 NF-κB 通路调节的炎症标志物的表达分析来评估其保护、增殖、抗氧化和抗炎作用。结果表明，用 预处理 hADMSCs 可提高 hADMSCs 的细胞活力、增殖和保护潜力，同时降低 LDH、ROS，并提高 SOD 活性。此外，ELISA 和基因表达分析均表明，与应激相比，预处理的 hADMSCs 中炎症标志物（IL1-β、TNF-α、p65、p50、MMP13）的表达下调。这是第一项报道使用 MIA 诱导的氧化应激对抗 hADMSC 并研究 对应激 hADMSCs 影响的研究。这些研究的结果提供了证据表明， 通过下调炎症标志物在应激条件下保护 hADMSCs，从而提高 hADMSCs 的活力和增殖潜力，这对于移植目的至关重要。
