Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU) Varanasi, Varanasi, 221005, India.
Department of Chemistry, Institute of Applied Science and Humanities, GLA University, Mathura, 281406, India.
Mol Biotechnol. 2024 Aug;66(8):2016-2022. doi: 10.1007/s12033-023-00816-w. Epub 2023 Aug 10.
Banana peel waste is one of the major contributors in the issue raised from solid waste, however, it can be valorized effectively due to high content of cellulose and hemicellulose. Significant conversion of banana waste includes cellulolytic enzymes and bioenergy production. In the present study, bacterial cellulase was produced using raw banana peel and ripe banana peel under SSF. Additionally, impact of acid pretreatment was investigated as one of strategy to improve cellulolytic enzyme production. A comparative evaluation of raw and ripe banana peels showed that ripe banana peels showed better enzyme production after pretreatment with 0.5% dilute HCl acid. In the series of enhancement of the enzyme production, temperature and pH of the SSF medium were also investigated, and found temperature 35 °C and pH 6.0 were optimum to produce maximum 3.5-U/ml FPA, 39-U/ml BGL, and 54-U/ml EG in 18-h SSF incubation. The study presented eco-friendly waste management to produce industrial enzyme for its promising application in waste valorization and biorefinery area.
香蕉皮废弃物是固体废弃物问题的主要来源之一，但由于其富含纤维素和半纤维素，因此可以有效地加以利用。香蕉废弃物的主要转化途径包括纤维素酶和生物能源生产。在本研究中，采用生香蕉皮和熟香蕉皮在固态发酵（SSF）条件下生产细菌纤维素酶。此外，还研究了酸预处理作为提高纤维素酶生产的策略之一的影响。生香蕉皮和熟香蕉皮的比较评估表明，在 0.5%稀盐酸预处理后，熟香蕉皮显示出更好的酶产量。在提高酶产量的一系列研究中，还考察了固态发酵培养基的温度和 pH 值，结果发现，在 35°C 的温度和 pH 值 6.0 条件下，在 18 小时的固态发酵孵育中可以产生最大的 3.5-U/ml FPA、39-U/ml BGL 和 54-U/ml EG。本研究提出了一种环保的废物管理方法，以生产工业酶，用于废物增值和生物炼制领域的有前途的应用。
