无细胞体系合成高分子量小肠多肽
Cell-free synthesis of high-molecular-weight small intestinal polypeptides.
作者信息
McAllister G, Bailey D S
出版信息
Biochim Biophys Acta. 1986 Sep 25;861(1):197-200. doi: 10.1016/0005-2736(86)90580-8.
PMID:3756153
Abstract
An intrinsic membrane glycoprotein, Mr 131 000, is a major developmentally specific component of the neonatal guinea-pig small intestinal microvillar membrane. Such high-molecular-weight proteins are often difficult to translate in vitro. In this study we report a successful strategy for the identification of the primary translation product of this glycoprotein, a high-molecular-weight precursor polypeptide of approximate Mr 225 000.
摘要
一种分子量为131 000的内在膜糖蛋白，是新生豚鼠小肠微绒毛膜主要的发育特异性成分。这类高分子量蛋白质通常很难在体外进行翻译。在本研究中，我们报告了一种成功的策略，用于鉴定这种糖蛋白的初级翻译产物，即一种分子量约为225 000的高分子量前体多肽。
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