Anderson C W, Lewis J B, Atkins J F, Gesteland R F
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2756-60. doi: 10.1073/pnas.71.7.2756.
Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were further identified by analysis of tryptic peptides. Translation of RNA fractionated by sedimentation through sucrose gradients containing formamide demonstrated seven size classes of RNA, each of which programmed the synthesis of only one or two virus-specific polypeptides. Six of the virus-specific polypeptides were translated from RNAs much larger than expected for the size of the polypeptide.
从感染2型腺病毒的人组织培养细胞中提取的细胞质RNA，被用于在源自哺乳动物细胞的无细胞系统中指导蛋白质合成。通过聚丙烯酰胺凝胶电泳对蛋白质产物进行分析，发现了十种腺病毒特异性多肽。其中五种通过胰蛋白酶肽分析得到了进一步鉴定。对通过含有甲酰胺的蔗糖梯度沉降分级分离的RNA进行翻译，结果显示有七种大小类别的RNA，每一种只能指导合成一或两种病毒特异性多肽。六种病毒特异性多肽是从比该多肽大小预期要大得多的RNA翻译而来的。
