Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments, Sun Yat-Sen University, Guangzhou 510275, China; Department of Medical Equipment, Hospital of Jiangxi University of Traditional Chinese Medicine (Jiangxi Provincial Hospital of Traditional Chinese Medicine), Nanchang, Jiangxi 330000, China.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments, Sun Yat-Sen University, Guangzhou 510275, China; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510000, China.
Food Chem. 2024 Jan 15;431:137107. doi: 10.1016/j.foodchem.2023.137107. Epub 2023 Aug 6.
Rapid and quantitative biochemical analysis at points-of-need is imperative for food safety inspection. This work reports on: 1) a stand-alone smartphone-based "two-in-one" spectrophotometer (the SAFS) installed with a self-developed application (the SAFS-App) which can precisely collect both absorption spectra and fluorescence spectra in a reproducible manner within 5 s; and 2) a straightforward protocol for xanthine detection using fluorescent carbon nanodots and silver nanoparticles. The assay performed with the SAFS demonstrates high specificity towards xanthine, and a linear range of 1-60 μM with LODs of 0.38 and 0.58 μM for colorimetric and fluorometric readouts, respectively. The reliability and robustness of the SAFS are validated by on-site quantitation of xanthine in fish and serum samples, with comparable accuracy to HPLC method. More importantly, the SAFS presents itself as an appealing device which is accessible to everyone through the Internet of Things and can be tailored for diverse point-of-care testing applications.
在现场快速、定量地进行生化分析对于食品安全检测至关重要。本工作报道了：1）一种独立的基于智能手机的“二合一”分光光度计（SAFS），它配备了一个自行开发的应用程序（SAFS-App），可以在 5 秒内以可重复的方式精确地收集吸收光谱和荧光光谱；2）一种使用荧光碳纳米点和银纳米粒子检测黄嘌呤的简单方法。使用 SAFS 进行的测定对黄嘌呤具有很高的特异性，并且比色和荧光读数的线性范围分别为 1-60 μM，LOD 分别为 0.38 和 0.58 μM。通过现场定量测定鱼和血清样品中的黄嘌呤，验证了 SAFS 的可靠性和稳健性，其准确性可与 HPLC 方法相媲美。更重要的是，SAFS 是一种引人注目的设备，通过物联网可以为每个人所获得，并可针对各种即时检测应用进行定制。
