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环状SEC11A作为一种新型调节因子通过靶向ZHX2/GADD34轴调控碘-125放射性粒子诱导的肝癌抗癌作用的表征

Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis.

作者信息

Li Dong, Wang Wujie, Liu Bin, Jin Die, Wang Yang, He Guanghui, Guo Lei, Liu Wen, Li Yuliang

机构信息

Department of Interventional Medicine, The Second Hospital of Shandong University, Jinan, China.

Institute of Interventional Oncology, Shandong University, Jinan, China.

出版信息

Cell Death Discov. 2023 Aug 10;9(1):294. doi: 10.1038/s41420-023-01593-w.

DOI:10.1038/s41420-023-01593-w
PMID:37563132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10415397/
Abstract

Iodine-125 (I-125) radioactive seed implantation is used for the local treatment of hepatocellular carcinoma (HCC), but the molecular mechanisms regulating its anticancer effects remain incompletely understood. In this study, we report that hsa_circ_0000647 (circSEC11A) is highly expressed after I-125 treatment in HCC cell lines and tissues and is a key regulator of I-125-induced anticancer effects. CircSEC11A acts as a competing endogenous RNA (ceRNA) to sponge miR-3529-3p, promoting the expression of zinc fingers and homeoboxes 2 (ZHX2) and enhancing I-125-induced anticancer effects. Dual-luciferase reporter assay, RNA pull-down, RNA immunoprecipitation, and fluorescence in situ hybridization were thereafter performed to verify the interaction among the molecules. Anticancer effects were detected using CCK-8, flow cytometry, TUNEL, EdU, transwell, and wound healing assays. Furthermore, ZHX2 transcriptionally inhibits GADD34, a negative regulator of endoplasmic reticulum stress (ERS), to enhance I-125- induced anticancer effects in vivo and in vitro. In conclusion, we characterized circSEC11A as a novel regulator of I-125-induced anticancer effects in HCC via miR-3529-3p/ZHX2/GADD34 axis-mediated ERS. Thus, circSEC11A may act as a potential therapeutic target for I-125 implantation in the clinic.

摘要

碘-125（I-125）放射性粒子植入术用于肝细胞癌（HCC）的局部治疗，但其抗癌作用的分子机制仍未完全明确。在本研究中，我们发现hsa_circ_0000647（circSEC11A）在I-125处理后的HCC细胞系和组织中高表达，是I-125诱导抗癌作用的关键调节因子。CircSEC11A作为竞争性内源性RNA（ceRNA），吸附miR-3529-3p，促进锌指同源框2（ZHX2）的表达，增强I-125诱导的抗癌作用。随后进行双荧光素酶报告基因检测、RNA下拉实验、RNA免疫沉淀实验和荧光原位杂交实验，以验证分子间的相互作用。采用CCK-8、流式细胞术、TUNEL、EdU、Transwell和伤口愈合实验检测抗癌效果。此外，ZHX2转录抑制内质网应激（ERS）的负调节因子GADD34，以增强I-125在体内外诱导的抗癌作用。总之，我们将circSEC11A鉴定为通过miR-3529-3p/ZHX2/GADD34轴介导的ERS在I-125诱导的HCC抗癌作用中的新型调节因子。因此，circSEC11A可能成为临床上I-125植入治疗的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/98f107bf907e/41420_2023_1593_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/28034081d5f0/41420_2023_1593_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/9e8c8a80f304/41420_2023_1593_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/13cdc8f09d1b/41420_2023_1593_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/dfadfbc01da4/41420_2023_1593_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/025ddd9712c2/41420_2023_1593_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/485410be1293/41420_2023_1593_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/606322632e2e/41420_2023_1593_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/98f107bf907e/41420_2023_1593_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/28034081d5f0/41420_2023_1593_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/9e8c8a80f304/41420_2023_1593_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/13cdc8f09d1b/41420_2023_1593_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/dfadfbc01da4/41420_2023_1593_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/025ddd9712c2/41420_2023_1593_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/485410be1293/41420_2023_1593_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/606322632e2e/41420_2023_1593_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/10415397/98f107bf907e/41420_2023_1593_Fig8_HTML.jpg

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本文引用的文献

1
The Emerging Value of Circular Noncoding RNA Research in Cancer Diagnosis and Treatment.环状非编码 RNA 研究在癌症诊断和治疗中的新兴价值。
Cancer Res. 2023 Mar 15;83(6):809-813. doi: 10.1158/0008-5472.CAN-22-3014.
2
IGF2BP2-induced circRUNX1 facilitates the growth and metastasis of esophageal squamous cell carcinoma through miR-449b-5p/FOXP3 axis.IGF2BP2 诱导的 circRUNX1 通过 miR-449b-5p/FOXP3 轴促进食管鳞癌细胞的生长和转移。
J Exp Clin Cancer Res. 2022 Dec 15;41(1):347. doi: 10.1186/s13046-022-02550-8.
3
ZHX2 in health and disease.
锌指蛋白2在健康与疾病中的作用。 （注：这里根据常见医学领域对ZHX2的理解进行翻译，ZHX2是锌指蛋白2（zinc finger homeobox 2）的缩写 ）
Front Oncol. 2022 Nov 18;12:1038890. doi: 10.3389/fonc.2022.1038890. eCollection 2022.
4
Application of Circular RNA Circ_0071662 in the Diagnosis and Prognosis of Hepatocellular Carcinoma and Its Response to Radiotherapy.环状 RNA Circ_0071662 在肝细胞癌的诊断和预后及其对放疗的反应中的应用。
Dig Dis. 2023;41(3):431-438. doi: 10.1159/000527696. Epub 2022 Oct 24.
5
USP13 promotes deubiquitination of ZHX2 and tumorigenesis in kidney cancer.USP13 促进肾癌细胞中 ZHX2 的去泛素化和肿瘤发生。
Proc Natl Acad Sci U S A. 2022 Sep 6;119(36):e2119854119. doi: 10.1073/pnas.2119854119. Epub 2022 Aug 29.
6
A Circ-0007022/miR-338-3p/Neuropilin-1 Axis Reduces the Radiosensitivity of Esophageal Squamous Cell Carcinoma by Activating Epithelial-To-Mesenchymal Transition and PI3K/AKT Pathway.环状RNA-0007022/微小RNA-338-3p/神经纤毛蛋白-1轴通过激活上皮-间质转化和PI3K/AKT通路降低食管鳞状细胞癌的放射敏感性
Front Genet. 2022 Apr 29;13:854097. doi: 10.3389/fgene.2022.854097. eCollection 2022.
7
Circular RNA and Its Roles in the Occurrence, Development, Diagnosis of Cancer.环状RNA及其在癌症发生、发展和诊断中的作用。
Front Oncol. 2022 Apr 7;12:845703. doi: 10.3389/fonc.2022.845703. eCollection 2022.
8
The function and regulation network mechanism of circRNA in liver diseases.环状RNA在肝脏疾病中的功能及调控网络机制
Cancer Cell Int. 2022 Mar 31;22(1):141. doi: 10.1186/s12935-022-02559-1.
9
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Ann Hepatol. 2022 Mar-Apr;27(2):100678. doi: 10.1016/j.aohep.2022.100678. Epub 2022 Jan 27.
10
Cancer statistics, 2022.癌症统计数据，2022 年。
CA Cancer J Clin. 2022 Jan;72(1):7-33. doi: 10.3322/caac.21708. Epub 2022 Jan 12.