Institute of Plant Biology and Biotechnology, Timiryazev 45, Almaty, Kazakhstan, 050040.
Department of Environmental Chemistry and Technology, Faculty of Environment, Jan Evangelista Purkyně University, Pasteurova 3632/15, 400 96, Usti nad Labem, Czech Republic.
Environ Sci Pollut Res Int. 2023 Sep;30(42):96098-96113. doi: 10.1007/s11356-023-29031-5. Epub 2023 Aug 11.
The effect of inoculation of Miscanthus × giganteus Greef et Deu by the plant growth promoting rhizobacteria (PGPRs) to the phytoremediation process and physio-biochemical plant's parameters was investigated in soil contaminated with the trace elements (TEs) from the Tekeli mining complex, Kazakhstan. Yeast Trichosporon sp. CA1, strains Rhizobium sp. Zn1-1, Shinella sp. Zn5-6, and Pseudomonas sp. CHA1-4, resistant to Zn and Pb, were isolated from the rhizosphere of M × g when the plant was cultivated in the same contaminated soil. Results illustrated that inoculation improved M × g adaptability to TEs toxicity by increasing the tolerance index to 2.9. The treatment enhanced the aboveground biomass yield by up to 163%, root biomass by up to 240%, chlorophyll content by up to 30%, and Chl ratio by up to 21%. Through M × g active growth and development, the peak activity of antioxidant enzymes was observed: activity of superoxide dismutase and glutathione reductase was induced, while the activity of catalase and ascorbate peroxidase was inhibited. Based on bioconcentration and translocation factors it was revealed that PGPRs selectively increased the uptake of TEs or stabilised them in the M × g rhizosphere. Inoculation with PGPRs increased the stabilization of Pb, V, Cr, Co, Ni, Cu, Cd, As, and Ba in the soil and plant tissues. Further research should focus on ex situ experiments using isolated PGPRs.
研究了接种植物促生根际细菌（PGPR）对受微量元素（TEs）污染土壤中芒属（Miscanthus × giganteus Greef et Deu）的植物修复过程和生理生化参数的影响。从哈萨克斯坦 Tekeli 矿区受污染土壤中芒属植物根际分离出耐锌和耐铅的酵母 Trichosporon sp. CA1、根瘤菌 Rhizobium sp. Zn1-1、鞘氨醇单胞菌 Shinella sp. Zn5-6 和假单胞菌 Pseudomonas sp. CHA1-4。结果表明，接种提高了 M × g 对 TEs 毒性的适应性，使耐量指数增加到 2.9。该处理将地上生物量提高了 163%，根生物量提高了 240%，叶绿素含量提高了 30%，Chl 比提高了 21%。通过 M × g 的积极生长和发育，观察到抗氧化酶的峰值活性：超氧化物歧化酶和谷胱甘肽还原酶的活性被诱导，而过氧化氢酶和抗坏血酸过氧化物酶的活性被抑制。基于生物浓缩和转运因子，发现 PGPRs 选择性地增加了 TEs 的吸收或在 M × g 根际稳定它们。接种 PGPRs 增加了 Pb、V、Cr、Co、Ni、Cu、Cd、As 和 Ba 在土壤和植物组织中的稳定性。进一步的研究应集中在使用分离的 PGPRs 进行的异位实验上。
