Key Lab of Organic-based Fertilizers of China, Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Key Lab of Organic-based Fertilizers of China, Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; School of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China.
Mol Plant. 2023 Sep 4;16(9):1379-1395. doi: 10.1016/j.molp.2023.08.004. Epub 2023 Aug 10.
The RIPENING-INHIBITOR (RIN) transcriptional factor is a key regulator governing fruit ripening. While RIN also affects other physiological processes, its potential roles in triggering interactions with the rhizosphere microbiome and plant health are unknown. Here we show that RIN affects microbiome-mediated disease resistance via root exudation, leading to recruitment of microbiota that suppress the soil-borne, phytopathogenic Ralstonia solanacearum bacterium. Compared with the wild-type (WT) plant, RIN mutants had different root exudate profiles, which were associated with distinct changes in microbiome composition and diversity. Specifically, the relative abundances of antibiosis-associated genes and pathogen-suppressing Actinobacteria (Streptomyces) were clearly lower in the rhizosphere of rin mutants. The composition, diversity, and suppressiveness of rin plant microbiomes could be restored by the application of 3-hydroxyflavone and riboflavin, which were exuded in much lower concentrations by the rin mutant. Interestingly, RIN-mediated effects on root exudates, Actinobacteria, and disease suppression were evident from the seedling stage, indicating that RIN plays a dual role in the early assembly of disease-suppressive microbiota and late fruit development. Collectively, our work suggests that, while plant disease resistance is a complex trait driven by interactions between the plant, rhizosphere microbiome, and the pathogen, it can be indirectly manipulated using "prebiotic" compounds that promote the recruitment of disease-suppressive microbiota.
RIPENING-INHIBITOR（RIN）转录因子是调控果实成熟的关键调节因子。虽然 RIN 也影响其他生理过程，但它在触发与根际微生物组和植物健康相互作用中的潜在作用尚不清楚。在这里，我们表明 RIN 通过根系分泌物影响微生物介导的抗病性，导致招募抑制土传、植物病原性罗尔斯顿氏菌的微生物群。与野生型（WT）植物相比，RIN 突变体具有不同的根系分泌物谱，这与微生物组组成和多样性的明显变化有关。具体而言，抗生素相关基因和抑制病原体的放线菌（链霉菌）的相对丰度在 rin 突变体的根际明显较低。通过应用 3-羟基黄酮和核黄素可以恢复 rin 植物微生物组的组成、多样性和抑制性，rin 突变体分泌的这两种物质的浓度要低得多。有趣的是，RIN 对根分泌物、放线菌和疾病抑制的介导作用从幼苗期就很明显，这表明 RIN 在早期形成抑制疾病的微生物组和后期果实发育中发挥双重作用。总的来说，我们的工作表明，虽然植物抗病性是由植物、根际微生物组和病原体之间的相互作用驱动的复杂特征，但可以使用“益生元”化合物间接操纵它，这些化合物可以促进抑制疾病的微生物组的招募。
