口腔潜在恶性疾病和口腔癌中爱泼斯坦-巴尔病毒感染的分析：一项横断面研究。

Analysis of Epstein-Barr Virus Infection in Oral Potentially Malignant Disorders and Oral Cancer: A Cross-Sectional Study.

作者信息

Pankam Jintana, Lapthanasupkul Puangwan, Kitkumthorn Nakarin, Rungraungrayabkul Dulyapong, Klongnoi Boworn, Piboonniyom Khovidhunkit Siribang-On

机构信息

Development of Disease Management Model for Oral Cancer with an Integration Network of Screening, Surveillance, and Treatment from Primary Care Unit to Tertiary Care in Nakhon Ratchasima Province Project, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

出版信息

J Int Soc Prev Community Dent. 2023 Jun 29;13(3):221-228. doi: 10.4103/jispcd.JISPCD_235_22. eCollection 2023 May-Jun.

DOI:10.4103/jispcd.JISPCD_235_22

PMID:37564166

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10411295/

Abstract

AIMS AND OBJECTIVES

The primary objective of this study is to determine the prevalence of Epstein-Barr virus (EBV) in oral potentially malignant disorders (OPMDs) and oral cancer (OC) in a group of Thais using polymerase chain reaction (PCR) and Epstein-Barr encoding regions () hybridization (ISH). The secondary objective is to investigate the risk factors of OC and the association between the presence of EBV and risk factors of OC/site of oral lesions.

MATERIALS AND METHODS

Sixty-one participants attending the screening project for OC and OPMDs at the Northeastern district hospitals of Thailand were recruited. Information related to risk factors and biopsy tissues for histopathological diagnosis was collected. Sixty-seven paraffin tissue blocks, including 52 OPMDs and 15 OC specimens, were investigated for EBV infection, using PCR analysis with latent membrane protein-1 () primer and ISH. Pearson's Chi-square or Fisher's exact test was used to analyze the differences in variables between participants with OPMDs and OC, as appropriate. The association between EBV infection and related risk factors was analyzed using logistic regression with a significant level at 0.05.

RESULTS

Using PCR analysis, 8 of 67 specimens (11.94%) were positive for Three cases of OPMDs were positive for both PCR and ISH. Regarding risk factors of OC, the two most common risk factors were betel nut chewing (52.46%) and working in sunlight (42.62%). The habit of taking alcohol was significantly different between the OC and the OPMDs groups ( = 0.009). The association between and the lesion at the tongue was statistically significant, with odds ratio = 4.900 (95% confidence interval = 1.046-22.943; = 0.044).

CONCLUSIONS

The prevalence of EBV infection in this group of participants was low. However, OPMDs at the tongue exhibited a significant association with EBV infection.

摘要

目的

本研究的主要目的是采用聚合酶链反应（PCR）和爱泼斯坦 - 巴尔编码区原位杂交（ISH）技术，确定一组泰国人中口腔潜在恶性疾病（OPMDs）和口腔癌（OC）中爱泼斯坦 - 巴尔病毒（EBV）的流行率。次要目的是调查口腔癌的危险因素以及EBV的存在与口腔癌危险因素/口腔病变部位之间的关联。

材料与方法

招募了61名在泰国东北部地区医院参加口腔癌和口腔潜在恶性疾病筛查项目的参与者。收集了与危险因素和用于组织病理学诊断的活检组织相关的信息。使用针对潜伏膜蛋白-1（LMP-1）引物的PCR分析和原位杂交技术，对67个石蜡组织块进行研究，其中包括52个口腔潜在恶性疾病标本和15个口腔癌标本。根据情况，使用Pearson卡方检验或Fisher精确检验分析口腔潜在恶性疾病参与者和口腔癌参与者之间变量的差异。使用逻辑回归分析EBV感染与相关危险因素之间的关联，显著性水平设定为0.05。

结果

通过PCR分析，67个标本中有8个（11.94%）LMP-1呈阳性。3例口腔潜在恶性疾病同时PCR和原位杂交均呈阳性。关于口腔癌的危险因素，两个最常见的危险因素是嚼槟榔（52.46%）和在阳光下工作（42.62%）。口腔癌组和口腔潜在恶性疾病组之间饮酒习惯有显著差异（P = 0.009）。LMP-1与舌部病变之间的关联具有统计学意义，优势比 = 4.900（95%置信区间 = 1.046 - 22.943；P = 0.044）。