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对比不同洋葱（Allium cepa L.）基因型在干旱胁迫下的转录组分析。

Comparative transcriptome analyses in contrasting onion (Allium cepa L.) genotypes for drought stress.

机构信息

ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, India.

Department of Bioinformatics, Savitribai Phule Pune University, Pune, India.

出版信息

PLoS One. 2020 Aug 11;15(8):e0237457. doi: 10.1371/journal.pone.0237457. eCollection 2020.

DOI:10.1371/journal.pone.0237457
PMID:32780764
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7418993/
Abstract

Onion (Allium cepa L.) is an important vegetable crop widely grown for diverse culinary and nutraceutical properties. Being a shallow-rooted plant, it is prone to drought. In the present study, transcriptome sequencing of drought-tolerant (1656) and drought-sensitive (1627) onion genotypes was performed to elucidate the molecular basis of differential response to drought stress. A total of 123206 and 139252 transcripts (average transcript length: 690 bases) were generated after assembly for 1656 and 1627, respectively. Differential gene expression analyses revealed upregulation and downregulation of 1189 and 1180 genes, respectively, in 1656, whereas in 1627, upregulation and downregulation of 872 and 1292 genes, respectively, was observed. Genes encoding transcription factors, cytochrome P450, membrane transporters, and flavonoids, and those related to carbohydrate metabolism were found to exhibit a differential expression behavior in the tolerant and susceptible genotypes. The information generated can facilitate a better understanding of molecular mechanisms underlying drought response in onion.

摘要

洋葱（Allium cepa L.）是一种重要的蔬菜作物，因其多样的烹饪和营养特性而广泛种植。作为一种浅根植物，它容易受到干旱的影响。在本研究中，对耐旱（1656）和干旱敏感（1627）洋葱基因型进行了转录组测序，以阐明对干旱胁迫的差异响应的分子基础。分别对 1656 和 1627 进行组装后，共生成了 123206 和 139252 个转录本（平均转录本长度：690 个碱基）。差异基因表达分析表明，在 1656 中分别有 1189 和 1180 个基因上调和下调，而在 1627 中，分别有 872 和 1292 个基因上调和下调。发现编码转录因子、细胞色素 P450、膜转运蛋白和类黄酮以及与碳水化合物代谢相关的基因在耐旱和敏感基因型中表现出不同的表达行为。生成的信息可以促进对洋葱干旱响应分子机制的更好理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/e99269247924/pone.0237457.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/05e6ff59f43d/pone.0237457.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/295616232856/pone.0237457.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/9e9cbe8c1878/pone.0237457.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/1d29297ca5aa/pone.0237457.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/e99269247924/pone.0237457.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/c3b0ad5e7603/pone.0237457.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/b5bb132db877/pone.0237457.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/05e6ff59f43d/pone.0237457.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/295616232856/pone.0237457.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/9e9cbe8c1878/pone.0237457.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/1d29297ca5aa/pone.0237457.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/afe1/7418993/e99269247924/pone.0237457.g007.jpg

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