Department of Agronomy, National Taiwan University, Taipei, Taiwan.
Academia Sinica Biotechnology Center in Southern Taiwan, Tainan, Taiwan.
Plant Signal Behav. 2020;15(2):1722447. doi: 10.1080/15592324.2020.1722447. Epub 2020 Feb 5.
Global water shortage seriously threatens rice growth especially in irrigated production areas. Association of plants with beneficial soil microbes is one strategy for plant adaption to environmental stresses. In this study, rice ( L.) plants were colonized by the beneficial root-colonizing endophytic fungus (). We demonstrate that grain yield were higher in a-colonized rice plants compared to the uncolonized plants grown in soil. Moreover, effect on improving water stress tolerance in rice and its physiological mechanism were investigated in a hydroponic culture system. Polyethylene glycol (PEG) was applied to the culture solution to conduct the water stress condition. Water stress-induced leaf wilting and impairments in photosynthetic efficiency were diminished in -colonized plants. Furthermore, colonization promotes stomata closure and increases the leaf surface temperature under water stress. The malondialdehyde level (as an indicator for oxidative stress) was lower and the reduced to oxidized glutathione ratio was higher in -colonized and PEG-exposed rice plants compared to the uncolonized plants. Furthermore, the activities of the antioxidant enzymes catalase and glutathione reductase were up-regulated in inoculated rice seedlings under water stress. In conclusion, promotes rice performance under water stress by stomata closure and lower oxidative stress.
全球水资源短缺严重威胁着水稻的生长，特别是在灌溉生产区。植物与有益土壤微生物的联合是植物适应环境胁迫的一种策略。在这项研究中，水稻（ L.）植物被有益的根定殖内生真菌（）定殖。我们证明，与未定殖的植物相比，在土壤中生长的定殖植物的谷物产量更高。此外，我们还在水培培养系统中研究了对提高水稻水分胁迫耐受性及其生理机制的影响。聚乙二醇（PEG）被应用于培养液中以进行水分胁迫条件。在定殖植物中，水分胁迫诱导的叶片萎蔫和光合作用效率的损伤减轻。此外，定殖促进气孔关闭，并在水分胁迫下增加叶片表面温度。与未定殖的植物相比，定殖和 PEG 处理的水稻叶片中的丙二醛水平（作为氧化应激的指标）较低，还原型谷胱甘肽与氧化型谷胱甘肽的比值较高。此外，在水分胁迫下，接种的水稻幼苗中的抗氧化酶过氧化氢酶和谷胱甘肽还原酶的活性被上调。总之，通过气孔关闭和降低氧化应激，促进了水稻在水分胁迫下的表现。
