Li Youyou, Xiao Meifang, Zhang Yun, Li Zihan, Bai Shijie, Su Haoxiang, Peng Ruoyan, Wang Gaoyu, Hu Xiaoyuan, Song Xinran, Li Xin, Tang Chuanning, Lu Gang, Yin Feifei, Zhang Peijun, Du Jiang
Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China.
Department of Clinical Laboratory, Center for Laboratory Medicine, Hainan Women and Children's Medical Center, Haikou, China.
Front Microbiol. 2023 Jul 26;14:1165839. doi: 10.3389/fmicb.2023.1165839. eCollection 2023.
Papillomaviruses (PVs) can cause hyperplasia in the skin and mucous membranes of humans, mammals, and non-mammalian animals, and are a significant risk factor for cervical and genital cancers.
Using next-generation sequencing (NGS), we identified two novel strains of papillomavirus, PV-HMU-1 and PV-HMU-2, in swabs taken from belugas () at Polar Ocean Parks in Qingdao and Dalian.
We amplified the complete genomes of both strains and screened ten belugas and one false killer whale () for the late gene (L1) to determine the infection rate. In Qingdao, 50% of the two sampled belugas were infected with PV-HMU-1, while the false killer whale was negative. In Dalian, 71% of the eight sampled belugas were infected with PV-HMU-2. In their L1 genes, PV-HMU-1 and PV-HMU-2 showed 64.99 and 68.12% amino acid identity, respectively, with other members of . Phylogenetic analysis of combinatorial amino acid sequences revealed that PV-HMU-1 and PV-HMU-2 clustered with other known dolphin PVs but formed distinct branches. PVs carried by belugas were proposed as novel species under .
The discovery of these two novel PVs enhances our understanding of the genetic diversity of papillomaviruses and their impact on the beluga population.
乳头瘤病毒（PVs）可导致人类、哺乳动物和非哺乳动物的皮肤及黏膜增生，是宫颈癌和生殖系统癌症的重要风险因素。
我们利用下一代测序（NGS）技术，在青岛和大连极地海洋公园的白鲸拭子样本中鉴定出两种新型乳头瘤病毒毒株，即PV-HMU-1和PV-HMU-2。
我们扩增了两种毒株的完整基因组，并对白鲸和1头伪虎鲸的晚期基因（L1）进行了筛查，以确定感染率。在青岛，所采样的两头白鲸中有50%感染了PV-HMU-1，而伪虎鲸呈阴性。在大连，所采样的8头白鲸中有71%感染了PV-HMU-2。在其L1基因中，PV-HMU-1和PV-HMU-2与其他海豚PVs成员的氨基酸同一性分别为64.99%和68.12%。对组合氨基酸序列的系统发育分析表明，PV-HMU-1和PV-HMU-2与其他已知的海豚PVs聚集在一起，但形成了不同的分支。白鲸携带的PVs被提议作为海豚PVs下的新物种。
这两种新型PVs的发现增进了我们对乳头瘤病毒遗传多样性及其对白鲸种群影响的理解。
