Vakili-Sadeghi Emad, Najafpour Alireza, Mohammadi Rahim
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2023;14(7):389-395. doi: 10.30466/vrf.2023.1972630.3682. Epub 2023 Jul 15.
The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: In sham group just, laparotomy was performed and in IS group, animals experienced a 3 hr period testicular IS. In IS/REP group, a 3 hr period of IS followed by a 3 hr period of testicular REP for left testis and a one-week testicular REP for right testis were done. In this group animals were gavaged by 1.00 mL normal saline 1 hr before the onset of IS. In IS/REP/ Prop group, the same procedures for IS/REP animals were followed as well as gavage of 1.00 mL Prop extract solution 1 hr before the onset of IS. Analyses of biochemistry, histology, inflammatory biomarkers and sperm parameters were carried out. In IS/REP/Prop group, nitric oxide synthase malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine in IS/REP/Prop group were significantly decreased and, superoxide dismutase, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase were significantly increased compared to the other animals. In IS/REP/Prop group, seminiferous tubules (with normal spermatogenesis) showed all stages of spermatogenic cells with plentiful spermatozoa. Tubular deterioration and atrophy and spermatogenic cell loss in were seen in a limited extent. The mean concentrations of Interleukin-1 beta and tumor necrosis factor alpha in IS/REP/Prop were significantly decreased. Sperm quality was significantly improved by Prop in IS/REP/Prop group. It was concluded that Prop could be supportive in diminishing IS/REP injury in testicular tissue exposed to ischemia.
采用大鼠睾丸扭转模型来评估蜂胶（Prop）对缺血再灌注（IS/REP）损伤的可能影响。将24只健康雄性Wistar大鼠随机分为四组（每组n = 6），动物经历双侧睾丸扭转，具体如下：假手术组仅进行剖腹手术，IS组动物经历3小时的睾丸缺血。在IS/REP组中，左侧睾丸进行3小时的缺血，随后进行3小时的睾丸再灌注，右侧睾丸进行为期一周的再灌注。在该组中，动物在缺血开始前1小时灌胃1.00 mL生理盐水。在IS/REP/Prop组中，采用与IS/REP组动物相同的程序，在缺血开始前1小时灌胃1.00 mL蜂胶提取液。进行了生物化学、组织学、炎症生物标志物和精子参数分析。与其他组动物相比，IS/REP/Prop组中的一氧化氮合酶、丙二醛、髓过氧化物酶和8-羟基-2-脱氧鸟嘌呤显著降低，超氧化物歧化酶、总谷胱甘肽、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽S-转移酶显著升高。在IS/REP/Prop组中，生精小管（生精正常）显示出具有丰富精子的生精细胞的各个阶段。仅在有限程度上观察到小管退化、萎缩和生精细胞丢失。IS/REP/Prop组中白细胞介素-1β和肿瘤坏死因子α的平均浓度显著降低。蜂胶显著改善了IS/REP/Prop组的精子质量。得出结论：蜂胶有助于减轻暴露于缺血的睾丸组织中的IS/REP损伤。
