Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats.

Buildup of reactive oxygen species during testicular torsion causes oxidative stress and ischemia-reperfusion (I/R) injury in testis. The purpose of this study was to investigate influence of β-cryptoxanthin (BCX) on I/R injury in testicular torsion/detorsion in mature rats. Thirty mature male Wistar rats were divided into five groups of six animals each, including sham group: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hr with a 720-degree rotation, I/R group: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hr with a 720-degree rotation, I/R/Oil group: In this group, a midline scrotum cut was performed, the testicles were taken out, ischemia was created for 2 hr with a 720-degree rotation, and at the end of ischemia 100 µL of corn oil (BCX solvent) was injected intraperitoneally, I/R/BCX10 group: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (10.00 µg kg) at the end of ischemia, and I/R/BCX40: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (40.00 µg kg) at the end of ischemia. Evaluations were based on histopathological and spermatological parameters and oxidative stress assessments. Histopathological spermatological and oxidative stress parameters values obtained from I/R/BCX40 were significantly different from those of other groups ( < 0.05). It could be concluded that BCX could ameliorate testicular injuries in acute testicular torsion/detorsion in mature rats.