Shoaei Parisa, Ranjbar Mohammad M, Tokhanbigli Samaneh, Ataei Behrouz, Alibakhshi Abbas, Haghjooy Javanmard Shaghayegh, Ahangarzadeh Shahrzad
Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.
Adv Biomed Res. 2023 Jun 28;12:153. doi: 10.4103/abr.abr_171_22. eCollection 2023.
The spike surface glycoprotein of SARS-CoV-2 is the essential protein in virus attachment to the target cell and cell entrance. As this protein contains immunodominant epitopes and is the main target for immune recognition, it is the critical target for vaccine and therapeutics development. In the current research, we analyzed the variability and mutations of the spike glycoprotein isolated from 72 COVID-19-positive patients from Iran's first three waves of disease.
The RNA was extracted from nasopharyngeal samples of confirmed COVID-19 cases and served as a template for cDNA synthesis and reverse transcriptase polymerase chain reaction. The reverse transcriptase polymerase chain reaction products of each sample were assembled and sequenced.
After analysis of 72 sequences, we obtained 46 single nucleotide polymorphisms, including 23 that produce amino acid changes. Our analysis showed that the most frequent mutation was the D614G (in the samples of the second and third waves).
Our findings suggest that developing effective vaccines requires identifying the predominant variants of SARS-CoV-2 in each community.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的刺突表面糖蛋白是病毒附着于靶细胞并进入细胞的关键蛋白。由于该蛋白含有免疫显性表位，是免疫识别的主要靶点，因此是疫苗和治疗药物开发的关键靶点。在当前研究中，我们分析了从伊朗前三波疫情中72例新冠肺炎阳性患者分离出的刺突糖蛋白的变异性和突变情况。
从确诊的新冠肺炎病例的鼻咽样本中提取RNA，并用作cDNA合成和逆转录聚合酶链反应的模板。对每个样本的逆转录聚合酶链反应产物进行组装和测序。
在分析72个序列后，我们获得了46个单核苷酸多态性，其中23个导致氨基酸变化。我们的分析表明，最常见的突变是D614G（在第二波和第三波样本中）。
我们的研究结果表明，开发有效的疫苗需要识别每个社区中SARS-CoV-2的主要变体。
