Mraovitch S, Lasbennes F, Calando Y, Seylaz J
Brain Res. 1986 Aug 13;380(1):42-53. doi: 10.1016/0006-8993(86)91427-7.
Electrical stimulation of the centromedian-parafascicular complex (CM-Pf) in anesthetized (chloralose) and paralyzed (tubocurarine) rats elicits a widespread cerebrovascular dilatation. Regional cerebral blood flow (rCBF) was measured in dissected tissue samples of 10 brain regions (medulla, pons, cerebellum, inferior colliculus, superior colliculus, frontal parietal and occipital cortices, caudate-putamen and corpus callosum) by [14C]iodoantipyrine method. In unstimulated and sham-operated rats rCBF ranged from 40 +/- 3 (ml/100 g/min) in corpus callosum to 86 +/- 6 (ml/100 g/min) in inferior colliculus. During CM-Pf stimulation, rCBF increased significantly (P less than 0.05, analysis of variance and Scheffe's test) in all cerebral regions bilaterally ranging from +118% in parietal cortex to +38% in cerebellum. Although cerebral vasodilation elicited by CM-Pf stimulation persisted after unilateral transection of the cervical sympathetic trunk, the cortical CBF was significantly reduced (P less than 0.05) on the denervated side. Acute adrenalectomy significantly (P less than 0.05) decreased elevated rCBF during CM-Pf stimulation in all cortical regions (frontal-36%, parietal -34%, and occipital -27%) and in caudate nucleus (-37%). Thus, excitation of neurons originating in, or fibers passing through the CM-Pf can elicit a powerful cerebral vasodilation. The cerebral vasodilation is modulated by cervical sympathectomy and circulating adrenal hormones. We conclude that CM-Pf elicited vasodilation is at least partly mediated by intrinsic neural pathways.
在麻醉（水合氯醛）且瘫痪（筒箭毒碱）的大鼠中，对中央中-束旁复合体（CM-Pf）进行电刺激会引发广泛的脑血管扩张。通过[14C]碘安替比林法，在10个脑区（延髓、脑桥、小脑、下丘、上丘、额顶叶和枕叶皮质、尾状核-壳核以及胼胝体）的解剖组织样本中测量局部脑血流量（rCBF）。在未受刺激和假手术的大鼠中，rCBF范围从胼胝体的40±3（毫升/100克/分钟）到下丘的86±6（毫升/100克/分钟）。在CM-Pf刺激期间，双侧所有脑区的rCBF均显著增加（P<0.05，方差分析和谢费检验），范围从顶叶皮质的+118%到小脑的+38%。尽管在单侧切断颈交感干后，CM-Pf刺激引发的脑血管扩张仍然持续，但去神经侧的皮质CBF显著降低（P<0.05）。急性肾上腺切除术显著（P<0.05）降低了CM-Pf刺激期间所有皮质区域（额叶-36%、顶叶-34%和枕叶-27%）以及尾状核（-37%）中升高的rCBF。因此，起源于CM-Pf或通过CM-Pf的纤维的神经元兴奋可引发强烈的脑血管扩张。脑血管扩张受颈交感神经切除术和循环肾上腺激素的调节。我们得出结论，CM-Pf引发的血管扩张至少部分由内在神经通路介导。
