Mingmanit Yonlada, Boonsrangsom Thanita, Sujipuli Kawee, Ratanasut Kumrop, Inthima Phithak
Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, 99 Moo 9, Tha Pho, Phitsanulok 65000, Thailand.
Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, 99 Moo 9, Tha Pho, Phitsanulok 65000, Thailand.
AoB Plants. 2023 Jul 31;15(4):plad052. doi: 10.1093/aobpla/plad052. eCollection 2023 Jul.
Banana ( spp.) is a major global economic fruit crop. However, cross-pollination from other cultivars grown in nearby plantations results in seeded fruit that exceeds market demand. This study investigated pollen viability and germination and examined the expression profiles of pollen development-related genes across seven genomes (AA, BB, AAA, BBB, AAB, ABB and ABBB). Twenty-three cultivars were assessed for pollen viability using lacto-aceto-orcein and triphenyltetrazolium chloride staining methods. Results revealed that pollen viability obtained from both methods was significantly different among all the studied cultivars. Cultivars carrying BB (diploid) genomes had higher viability percentages than AA (diploid), AAA, BBB, AAB and ABB (triploid) and ABBB (tetraploid) genomes. Germination of the studied cultivars was also investigated on pollen culture medium, with results showing significant differences between the pollen of each cultivar. The best germinating cultivar was TKM (11.0 %), carrying BB genome. Expression profiles of pollen development-related genes by RT-qPCR indicated that both and genes were highly expressed in triploid genomes but the gene showed down-regulated expression, resulting in non-viable pollen. Pollen viability, pollen germination and pollen development-related genes differed across cultivars. This knowledge will be useful for the selection of male parents for cross-breeding programs. Pollen viability should also be considered when planning production to avoid seeded fruit.
香蕉（品种）是一种主要的全球经济水果作物。然而，附近种植园种植的其他品种的异花授粉会导致结出的种子果实超出市场需求。本研究调查了花粉活力和萌发情况，并检测了七个基因组（AA、BB、AAA、BBB、AAB、ABB和ABBB）中花粉发育相关基因的表达谱。使用乳酸-醋酸洋红和氯化三苯基四氮唑染色方法对23个品种的花粉活力进行了评估。结果显示，所有研究品种中，两种方法获得的花粉活力存在显著差异。携带BB（二倍体）基因组的品种的花粉活力百分比高于AA（二倍体）、AAA、BBB、AAB和ABB（三倍体）以及ABBB（四倍体）基因组。还在花粉培养基上研究了所研究品种的萌发情况，结果表明每个品种的花粉之间存在显著差异。萌发最好的品种是携带BB基因组的TKM（11.0%）。通过RT-qPCR对花粉发育相关基因的表达谱分析表明， 和 基因在三倍体基因组中高度表达，但 基因表达下调，导致花粉无活力。不同品种的花粉活力、花粉萌发和花粉发育相关基因存在差异。这些知识将有助于为杂交育种计划选择雄性亲本。在规划生产时也应考虑花粉活力，以避免结出有籽果实。
