Barroso-Arévalo Sandra, Díaz-Frutos Marta, Domínguez Lucas, Sánchez-Vizcaíno José M
VISAVET Health Surveillance Center, Complutense University of Madrid , Madrid, Spain.
Department of Animal Health, Faculty of Veterinary, Complutense University of Madrid , Madrid, Spain.
Microbiol Spectr. 2023 Aug 11;11(5):e0068023. doi: 10.1128/spectrum.00680-23.
The apparition of new variants of severe acute respiratory syndrome coronavirus 2 and lineages is constantly happening because of the high viral mutation rate. Since numerous reverse zoonosis events have been reported so far, genomic surveillance should be conducted in susceptible species to evaluate potential adaptations that may trigger the apparition of new variants. Here, we evaluate the evolution of the infection in a cat naturally infected in parallel with its owner, performing a comparative phylogenetic analysis. Sequencing analysis showed that both were infected with the Omicron BA.5/BF.1 lineage and revealed the presence of nucleotide substitution in the viral genome recovered from the cat with respect to the viral genome from the human sample. This nucleotide substitution (C11897A) produced the amino acid change Orf1a: Q3878K. Therefore, genomic surveillance in the case of reverse zoonosis events is still necessary in order to control possible adaptations of the virus to other susceptible species. IMPORTANCE Genomic surveillance of pets for severe acute respiratory syndrome coronavirus 2 is important to monitor the emergence of new variants of the virus associated with these animals. Pets can serve as a potential reservoir for the virus, and their close contact with humans increases the risk of transmission. By conducting genomic surveillance in pets, it is possible to detect and track new variants early on, allowing for more effective control measures to be put in place. This can help prevent the spread of these variants to human populations and potentially mitigate the impact of the pandemic. Furthermore, it may also provide insight into the evolution and spread of the virus within the animal population.
由于严重急性呼吸综合征冠状病毒2的高病毒突变率，新变种和谱系的出现一直在持续发生。鉴于目前已报告了众多反向人畜共患病事件，应对易感物种进行基因组监测，以评估可能引发新变种出现的潜在适应性变化。在此，我们通过比较系统发育分析，评估了一只与其主人同时自然感染的猫的感染演变情况。测序分析表明，两者均感染了奥密克戎BA.5/BF.1谱系，并揭示从猫身上回收的病毒基因组相对于人类样本的病毒基因组存在核苷酸替换。这种核苷酸替换（C11897A）导致了氨基酸变化Orf1a：Q3878K。因此，在反向人畜共患病事件中进行基因组监测对于控制病毒对其他易感物种的可能适应性变化仍然是必要的。重要性 对宠物进行严重急性呼吸综合征冠状病毒2的基因组监测对于监测与这些动物相关的病毒新变种的出现至关重要。宠物可能成为病毒的潜在宿主，它们与人类的密切接触增加了传播风险。通过对宠物进行基因组监测，可以尽早发现和追踪新变种，从而能够采取更有效的控制措施。这有助于防止这些变种传播到人类群体，并可能减轻大流行的影响。此外，这也可能为了解病毒在动物群体中的演变和传播提供见解。
