Ayhan Asude, Efe Ekin, Fidan Pinar A, Efe Oguzhan E, Ates Eylem Gul, Sahinturk Fikret, Ayhan Selim
Department of Anaesthesiology and Reanimation, Baskent University School of Medicine, Ankara, Turkey.
Department of Histology and Embryology, Baskent University School of Medicine, Ankara, Turkey.
Birth Defects Res. 2023 Oct 15;115(17):1598-1607. doi: 10.1002/bdr2.2237. Epub 2023 Aug 11.
Sugammadex is a modified gamma-cyclodextrin that has been developed with the goal of reversing the steroidal neuromuscular blocking agents. The aim of the present study is to investigate the effects of different sugammadex doses on embryologic and neural tube development in an early-stage chick embryo model.
A total of 100 specific pathogen-free, fertilized domestic chicken eggs were randomly divided into five groups (n = 20, each), and placed in an automatic cycle incubator. The eggs in the "control (C)" group were incubated without administration of any drug till the end of the experiment. Sub-blastodermic administration of 0.9% NaCl as vehicle control (VC) and different doses of sugammadex solutions prepared with the latter [2 mg/mL (LD), 4 mg/mL (MD), 16 mg/mL (HD)] were performed at 30 hr of incubation. All embryos were removed from the eggs at 72 hr when they were expected to reach Hamburger-Hamilton (HH) stages 19-20, then they were fixed, and evaluated histo-morphologically.
Embryonic development was not observed in 11 eggs (1 in C, 1 in VC; 3 in LD, 3 in MD, and 3 in HD). All the developed embryos were compatible with the HH stages 19-20. A neural tube closure defect was detected in one embryo in the HD group. No statistically significant difference was found between the groups in terms of embryonic and neural tube developments.
No significant association was found between the drug and adverse outcomes; however, a trend with dosing was seen. Further studies are required before conclude on safety and extrapolate these results to human beings.
舒更葡糖钠是一种经过修饰的γ-环糊精，其研发目的是逆转甾体类神经肌肉阻滞剂的作用。本研究旨在探讨不同剂量舒更葡糖钠对早期鸡胚模型胚胎学和神经管发育的影响。
总共100枚无特定病原体的受精家鸡蛋被随机分为五组（每组n = 20），并置于自动循环孵化器中。“对照（C）”组的鸡蛋在不给予任何药物的情况下孵化直至实验结束。在孵化30小时时，进行胚盘下注射0.9%氯化钠作为溶剂对照（VC）以及用后者配制的不同剂量舒更葡糖钠溶液[2毫克/毫升（低剂量组，LD）、4毫克/毫升（中剂量组，MD）、16毫克/毫升（高剂量组，HD）]。当所有胚胎预计达到汉伯格-汉密尔顿（HH）第19 - 20阶段时，在72小时从鸡蛋中取出，然后进行固定，并进行组织形态学评估。
11枚鸡蛋（C组1枚、VC组1枚；LD组3枚、MD组3枚、HD组3枚）未观察到胚胎发育。所有发育的胚胎均与HH第19 - 20阶段相符。在HD组的一枚胚胎中检测到神经管闭合缺陷。各组在胚胎和神经管发育方面未发现统计学上的显著差异。
未发现该药物与不良结局之间存在显著关联；然而，观察到有剂量相关趋势。在得出安全性结论并将这些结果外推至人类之前，还需要进一步研究。
