Laboratorio de Biología de la Reproducción, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi," Universidad Autónoma de Yucatán, Mérida, México.
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México.
Metab Syndr Relat Disord. 2023 Nov;21(9):503-508. doi: 10.1089/met.2023.0042. Epub 2023 Aug 9.
To investigate the possible association between rs3480 and rs16835198 of the fibronectin type III domain containing 5 () and their haplotypes with the presence of type 2 diabetes mellitus (T2DM) in Maya-Mestizo women. We studied 547 postmenopausal women of Maya-Mestizo origin. The diagnosis of T2DM was based on the criteria of the American Diabetes Association. DNA was obtained from blood leukocytes. rs3480 and rs16835198 of were studied using real-time polymerase chain reaction allelic discrimination. Deviations from Hardy-Weinberg equilibrium and alleles differences, as well as genotype frequencies between groups, were assessed by tests. Using logistic regression analysis, the odds ratio and 95% confidence intervals were calculated to estimate the association between both polymorphisms of FNDC5/Irisin and the presence of T2DM. Pairwise linkage disequilibrium between polymorphisms was calculated by direct correlation , and haplotype analysis was conducted. We found that the G-allele of rs3480, as well as under a dominant model, this polymorphism was significantly associated with T2DM ( = 0.028 and = 0.003, respectively). Besides, one haplotype was associated with T2DM ( = 0.035). Our results suggest that the rs3480, and one haplotype formed by rs3480 and rs16835198 were associated with the risk of presenting T2DM in Maya-Mestizo women.
为了研究纤维连接蛋白 III 型结构域包含 5 号 () 基因 rs3480 和 rs16835198 及其单倍型与梅萨-梅斯蒂索女性 2 型糖尿病 (T2DM) 之间可能存在的关联。我们研究了 547 名绝经后梅萨-梅斯蒂索女性。T2DM 的诊断基于美国糖尿病协会的标准。从血液白细胞中提取 DNA。使用实时聚合酶链反应等位基因鉴别法研究 rs3480 和 rs16835198 。通过 检验评估 Hardy-Weinberg 平衡偏离和等位基因差异以及组间基因型频率。使用逻辑回归分析计算优势比和 95%置信区间，以估计 FNDC5/Irisin 这两个多态性与 T2DM 存在之间的关联。通过直接相关 计算多态性之间的连锁不平衡，并进行单倍型分析。我们发现 rs3480 的 G 等位基因，以及在显性模型下，该多态性与 T2DM 显著相关（ = 0.028 和 = 0.003，分别）。此外，一种单倍型与 T2DM 相关（ = 0.035）。我们的结果表明， 基因 rs3480 以及由 rs3480 和 rs16835198 形成的一种单倍型与梅萨-梅斯蒂索女性发生 T2DM 的风险相关。
