Department of Chemistry, Hemchandracharya North Gujarat University, Patan 384265, Gujarat, India.
Sanjivani College of Pharmaceutical Education and Research, Kopargaon 423603, Maharashtra, India.
Int J Biol Macromol. 2023 Oct 1;250:126268. doi: 10.1016/j.ijbiomac.2023.126268. Epub 2023 Aug 9.
This study holds significant importance as it explores the synthesis and characterization of two chitosan dibenzylideneacetone Schiff bases. Various analytical techniques, such as UV-visible spectroscopy, FTIR, XRD, TGA, DSC, SEM, and elemental analysis, were employed to thoroughly examine these derivatives. The antimicrobial activity of the chitosan derivatives was evaluated against a range of bacterial and fungal strains, while cytotoxicity tests were conducted on MCF-7, L-132, and VERO cell lines. In the antimicrobial tests, the chitosan derivatives exhibited remarkable antibacterial properties against S. aureus, E. coli, and Pseudomonas aeruginosa, as well as potent antifungal properties against Candida albicans and Aspergillus fumigatus. The cytotoxicity assessment revealed that the dibenzylideneacetone chitosan Schiff base (CHDBA) showed significant effectiveness against the L-132 cell line, surpassing the efficacy of doxorubicin by 2.44 times. Moreover, it exhibited substantial activity against the L-132 and MCF-7 cell lines, with IC values of 55.29 μg/mL and 185.8 μg/mL, respectively. Notably, none of the chitosan derivatives demonstrated cytotoxicity towards the normal cell line, indicating their non-toxic nature and safe usability. Based on these findings, it is evident that CHDBA holds promise for further development as a potential treatment option for breast cancer and lung cancer.
这项研究具有重要意义，因为它探索了两种壳聚糖二苄叉丙酮席夫碱的合成和表征。使用了各种分析技术，如 UV-可见光谱、FTIR、XRD、TGA、DSC、SEM 和元素分析，对这些衍生物进行了彻底的检查。对壳聚糖衍生物的抗菌活性进行了评估，以对抗一系列细菌和真菌菌株，同时对 MCF-7、L-132 和 VERO 细胞系进行了细胞毒性测试。在抗菌测试中，壳聚糖衍生物对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌表现出显著的抗菌特性，对白色念珠菌和烟曲霉也表现出很强的抗真菌特性。细胞毒性评估表明，二苄叉丙酮壳聚糖席夫碱（CHDBA）对 L-132 细胞系具有显著的疗效，比阿霉素的疗效高 2.44 倍。此外，它对 L-132 和 MCF-7 细胞系具有显著的活性，IC 值分别为 55.29 μg/mL 和 185.8 μg/mL。值得注意的是，壳聚糖衍生物对正常细胞系均无细胞毒性，表明其具有非毒性和安全可用性。基于这些发现，CHDBA 有望进一步开发成为治疗乳腺癌和肺癌的潜在治疗选择。
