Paoli Donatella, Gilio Barbara, Piroli Emanuela, Gallo Mariagrazia, Lombardo Francesco, Dondero Franco, Lenzi Andrea, Gandini Loredana
Department of Medical Pathophysiology, University of Rome La Sapienza, Rome, Italy.
Fertil Steril. 2009 Feb;91(2):414-9. doi: 10.1016/j.fertnstert.2007.11.084. Epub 2008 Mar 4.
To evaluate the presence of antisperm antibodies in testicular cancer patients 1 month after orchiectomy and before radiotherapy or chemotherapy.
Clinical study.
Department of andrology and seminology at a university hospital.
PATIENT(S): One hundred ninety patients with testicular cancer.
INTERVENTION(S): Determination of semen parameters and autoimmune reaction evaluated on the sperm surface and in blood serum.
MAIN OUTCOME MEASURE(S): Autoimmune reaction on the sperm surface by the direct immunobead test (IBT), and in blood serum by the indirect IBT and the gelatin agglutination test (GAT), was evaluated 1 month after orchiectomy and before beginning chemotherapy or radiotherapy.
RESULT(S): Of the 190 patients, 11 (5.8%) were positive for antisperm antibody by GAT. On indirect IBT, 3 of the 11 GAT-positive patients were positive to IgG class only, with values of 22%, 24%, and 40%. Of the 11 GAT-positive patients, 4 showed no antibody bound to the sperm surface, and 3 were positive to IgG class only (28%, 21%, and 38%), with binding exclusively on the tail. Direct IBT could not be performed in the remaining 4 patients.
CONCLUSION(S): Our data support the hypothesis that testicular cancer might not be a possible cause of antisperm autoimmunization and infertility.
评估睾丸癌患者在睾丸切除术后1个月以及放疗或化疗前抗精子抗体的存在情况。
临床研究。
某大学医院的男科与精液学科室。
190例睾丸癌患者。
测定精液参数以及评估精子表面和血清中的自身免疫反应。
在睾丸切除术后1个月以及开始化疗或放疗前，通过直接免疫珠试验（IBT）评估精子表面的自身免疫反应，通过间接IBT和明胶凝集试验（GAT）评估血清中的自身免疫反应。
190例患者中，11例（5.8%）GAT抗精子抗体呈阳性。在间接IBT中，11例GAT阳性患者中有3例仅IgG类呈阳性，值分别为22%、24%和40%。11例GAT阳性患者中，4例精子表面未显示抗体结合，3例仅IgG类呈阳性（28%、21%和38%），且仅尾部有结合。其余4例患者无法进行直接IBT。
我们的数据支持以下假设，即睾丸癌可能不是抗精子自身免疫和不育的可能原因。
