Han Xiangpeng, Song Ya, Huang Riming, Zhu Minqian, Li Meiying, Requena Teresa, Wang Hong
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Laboratory for Lingnan Mordern Agriculture, Guangzhou 510642, China.
Foods. 2023 Jul 29;12(15):2889. doi: 10.3390/foods12152889.
This study aimed to explore the anti-inflammatory and gut microbiota modulation potentials of flavonoid-rich fraction (PFF) extracted from fruits. The results showed that PFF markedly reduced the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in LPS-stimulated RAW 264.7 cells. Meanwhile, PFF treatment also effectively decreased the phosphorylation levels of MAPK, PI3K/Akt, and NF-κB signaling-pathway-related proteins (ERK, JNK, p38, Akt, and p65). Moreover, PFF had an impact on microbial composition and metabolites in a four-stage dynamic simulator of human gut microbiota (BFBL gut model). Specifically, PFF exhibited the growth-promoting ability of several beneficial bacteria, including , , , and , and short-chain fatty acid (SCFA) generation ability in gut microbiota. In addition, spectroscopic data revealed that PFF mainly contained five flavonoid compounds, which may be bioactive compounds with anti-inflammatory and gut microbiota modulation potentials. Therefore, PFF could be utilized as a natural anti-inflammatory agent or supplement to health products.
本研究旨在探索从水果中提取的富含黄酮类化合物的组分（PFF）的抗炎和调节肠道微生物群的潜力。结果表明，PFF显著降低了脂多糖刺激的RAW 264.7细胞中一氧化氮（NO）、肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）的产生。同时，PFF处理也有效降低了丝裂原活化蛋白激酶（MAPK）、磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）和核因子κB（NF-κB）信号通路相关蛋白（细胞外信号调节激酶（ERK）、c-Jun氨基末端激酶（JNK）、p38、Akt和p65）的磷酸化水平。此外，PFF对人类肠道微生物群的四阶段动态模拟器（BFBL肠道模型）中的微生物组成和代谢产物有影响。具体而言，PFF表现出对几种有益细菌（包括[此处缺失具体细菌名称]、[此处缺失具体细菌名称]、[此处缺失具体细菌名称]和[此处缺失具体细菌名称]）的促生长能力以及在肠道微生物群中产生短链脂肪酸（SCFA）的能力。此外，光谱数据显示PFF主要含有五种黄酮类化合物，它们可能是具有抗炎和调节肠道微生物群潜力的生物活性化合物。因此，PFF可作为一种天然抗炎剂或健康产品的补充剂。
